Development of an in vitro model to study clot lysis activity of thrombolytic drugs.
Development of an in vitro model to study clot lysis activity of thrombolytic drugs.
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DOI：
10.1186/1477-9560-4-14
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发表时间：
2006-09-12
影响因子：
3.1
通讯作者：
Daginawala HF
中科院分区：
文献类型：
--
作者：
Prasad S;Kashyap RS;Deopujari JY;Purohit HJ;Taori GM;Daginawala HF
溶栓药物是治疗脑静脉窦血栓形成的常用药物。目前已有多种体外模型用于研究溶栓药物的溶栓活性，但都存在一定的局限性。需要一个合适的模型来检验溶栓药物的溶栓效果。在本研究中，试图设计和开发一种新的模型系统，以研究血栓溶解在一个简单和容易的方式使用溶栓药物，链激酶。使来自健康个体（n = 20）的全血在预称重的无菌微量离心管中形成凝块;去除血清并称重凝块。用链激酶溶解后，除去液体，并再次沿着试管称量凝块残留物。根据凝块溶解前后获得的微量离心管的重量差异计算凝块溶解百分比。当使用链激酶时，观察到显著百分比的凝块溶解。另一方面，用水（阴性对照）时，观察到极轻微（2.5%）凝块溶解。链激酶和水溶解凝块的效果有显著差异。本研究为研究新药及已知药物的溶栓作用提供了一种快速有效的方法。
Thrombolytic drugs are widely used for the management of cerebral venous sinus thrombosis patients. Several in vitro models have been developed to study clot lytic activity of thrombolytic drugs, but all of these have certain limitations. There is need of an appropriate model to check the clot lytic efficacy of thrombolytic drugs. In the present study, an attempt has been made to design and develop a new model system to study clot lysis in a simplified and easy way using a thrombolytic drug, streptokinase. Whole blood from healthy individuals (n = 20) was allowed to form clots in a pre-weighed sterile microcentrifuge tubes; serum was removed and clot was weighed. After lysis by streptokinase fluid was removed and remnants of clot were again weighed along with the tube. Percentage of Clot lysis was calculated on the basis of the weight difference of microcentrifuge tubes obtained before and after clot lysis. There was a significant percentage of clot lysis observed when streptokinase was used. On the other hand with water (negative control), minimal (2.5%) clot lysis was observed. There was a significant difference between clot lysis done by streptokinase and water. Our study could be a rapid and effective methodology to study clot-lytic effect of newly developed drugs as well as known drugs.