PolyIC Induces Retinoic Acid-inducible Gene-I and Melanoma Differentiation-associated Gene 5 and Modulates Inflammation in Podocytes
PolyIC Induces Retinoic Acid-inducible Gene-I and Melanoma Differentiation-associated Gene 5 and Modulates Inflammation in Podocytes
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DOI：
10.21873/invivo.12242
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发表时间：
2021
期刊：
影响因子：
2.3
通讯作者：
Masamichi Nakata;M. Shimada;Ikuyo Narita-Kinjo;Daiki Nagawa;Kazutaka Kitayama;Misato Hamadate;Naotake Miura;Masashi Nozaka;Yosuke Kawamura;Takeshi Fujita;Reiichi Murakami;T. Imaizumi;Norio Nakamura;H. Tomita
中科院分区：
文献类型：
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作者：
Masamichi Nakata;M. Shimada;Ikuyo Narita-Kinjo;Daiki Nagawa;Kazutaka Kitayama;Misato Hamadate;Naotake Miura;Masashi Nozaka;Yosuke Kawamura;Takeshi Fujita;Reiichi Murakami;T. Imaizumi;Norio Nakamura;H. Tomita
背景/目的：病毒感染常使蛋白尿加重，这已被认为是由于足细胞的抗病毒反应。我们研究了多聚肌苷酸-多聚胞苷酸（polyIC）对培养的分化的人足细胞中维甲酸诱导基因-I（RIG-I）和黑素瘤分化相关基因5（MDA 5）表达的影响。材料与方法：将足细胞用2ng/ml至500 μg/ml的polyIC处理3至36小时，并且还用针对RIG-I和MDA 5的siRNA转染。进行F-肌动蛋白染色以评估肌动蛋白重组。结果如下：PolyIC以剂量和时间依赖性方式诱导RIG-I和MDA 5的表达，并伴随干扰素-β（IFN-β）和白细胞介素-6（IL-6）的上调和肌动蛋白重组。通过siRNA暂时敲低RIG-I降低IFN-β表达，而MDA 5 siRNA抑制IFN-β和IL-6表达。肌动蛋白重组减弱RIG-I和MDA 5敲低。结论：RIG-I和MDA 5可能参与足细胞的抗病毒反应。
Background/Aim: Viral infection often exacerbates proteinuria, which has been suggested to be due to antiviral responses of podocytes. We examined the effect of polyinosinic-polycytidylic acid (polyIC) on the expression of retinoic acid-inducible gene-I (RIG-I) and melanoma differentiation-associated gene 5 (MDA5) in differentiated human podocytes in culture. Materials and Methods: The podocytes were treated with 2 ng/ml to 500 μg/ml of polyIC for 3 to 36 h, and also transfected with siRNA against RIG-I and MDA5. F-actin staining was performed to assess actin reorganization. Results: PolyIC induced the expression of RIG-I and MDA5 in dose- and time-dependent manner, accompanied with interferon-β (IFN-β) and interleukin-6 (IL-6) up-regulation and actin reorganization. Temporal knockdown of RIG-I by siRNA decreased IFN-β expression, while MDA5 siRNA inhibited IFN-β and IL-6 expression. Actin reorganization was attenuated by RIG-I and MDA5 knockdown. Conclusion: RIG-I and MDA5 may play a role in the antiviral responses of podocytes.