Glyoxalase Centennial conference: introduction, history of research on the glyoxalase system and future prospects
Glyoxalase Centennial conference: introduction, history of research on the glyoxalase system and future prospects
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DOI：
10.1042/bst20140014
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发表时间：
2014-04-01
影响因子：
3.9
通讯作者：
Thornalley, Paul J.
中科院分区：
文献类型：
--
作者：
Rabbani, Naila;Thornalley, Paul J.
2013年11月27日至29日，研究人员聚集在英国考文垂华威大学，庆祝乙二醛酶途径发现一百周年。乙二醛酶系统是由卡尔·纽伯格、亨利·德赖斯代尔·达金和哈罗德·沃德·达德利于1913年在论文中发现并报告的。在生物化学的先驱年代，他们三人都是杰出的领军人物。Neuberg提出乙二醛酶是主流糖酵解的途径，Gustav Embden正确地否定了这一点，后来被Otto Meyerhof证实。Albert Szent-Gyorgyi提出乙二醛酶I是细胞生长的调节剂，而其他人对此表示怀疑。同时，对该体系的酶组分乙二醛酶I和乙二醛酶II的分子、结构和机理特性进行了表征。乙二醛酶途径的生理功能防御二羰基糖化，特别是内源性甲基乙二醛，现在似乎是安全的。我们现在正处于一个研究乙二醛酶系统调控的时代，它在衰老和疾病、生理应激和耐药性以及健康食品和新药物的开发中发挥着重要作用。乙二醛酶研究的历史说明了提出假设、测试和拒绝或接受进一步调查的科学过程，证明了需要以经验和专业知识为指导的直觉，以及不知疲倦的实验。
On 27-29 November 2013, researchers gathered at the University of Warwick, Coventry, U.K., to celebrate the centennial of the discovery of the glyoxalase pathway. The glyoxalase system was discovered and reported in papers by Carl Neuberg and by Henry Drysdale Dakin and Harold Ward Dudley in 1913. All three were leading extraordinary investigators in the pioneering years of biochemistry. Neuberg proposed glyoxalase as the pathway of mainstream glycolysis and Gustav Embden correctly discounted this, later confirmed by Otto Meyerhof. Albert Szent-Gyorgyi proposed glyoxalase I as the regulator of cell growth and others discounted this. In the meantime, molecular, structural and mechanistic properties of the enzymatic components of the system, glyoxalase I and glyoxalase II, have been characterized. The physiological function of the glyoxalase pathway of enzymatic defence against dicarbonyl glycation, particularly by endogenous methylglyoxal, now seems secure. We are now in an era of investigation of the regulation of the glyoxalase system where a role in aging and disease, physiological stress and drug resistance and development of healthier foods and new pharmaceuticals is emerging. The history of glyoxalase research illustrates the scientific process of hypothesis proposal, testing and rejection or acceptance with further investigation, standing testament to the need for intuition guided by experience and expertise, as well as indefatigable experimentation.