Prevalence and genetic characteristics of Cryptosporidium, Enterocytozoon bieneusi and Giardia duodenalis in cats and dogs in Heilongjiang province, China
Prevalence and genetic characteristics of Cryptosporidium, Enterocytozoon bieneusi and Giardia duodenalis in cats and dogs in Heilongjiang province, China
复制标题
黑龙江省猫犬隐孢子虫、比氏肠细胞虫和十二指肠贾第鞭毛虫的流行状况及遗传特征
DOI：
10.1016/j.vetpar.2015.01.014
复制
发表时间：
2015-03-15
影响因子：
2.6
通讯作者：
Xiao, Lihua
中科院分区：
文献类型：
--
作者：
Li, Wei;Li, Yijing;Xiao, Lihua
本文调查了黑龙江省中国地区猫、犬共319份粪便标本中隐孢子虫、毕氏肠胞原虫、十二指肠贾第鞭毛虫的感染情况及遗传特征。小亚基rRNA基因的聚合酶链式反应和DNA序列分析分别检出1只猫(3.8%)和1只猫(3.8%)，6只狗(2.2%)检出犬和泛囊线虫。核糖体内转录间隔区的多态性和系统发育分析表明，人畜共患埃希氏菌基因D、EbpC、NED1和NED2，宿主适应基因NED3、Ned4和PtEb IX，18只狗(6.7%)，3只猫(5.8%)人类致病基因D和IV。根据十二指肠球虫磷酸丙糖异构酶基因(TPI)的超突变进行基因分型，可以鉴定出1只猫的F组合(1.9%)和12只狗的C和E组合(4.5%)。通过测定TPI核苷酸和氨基酸序列的多样性来确定十二指肠吸虫分离株的亚型。在中国的人感染中发现了犬肠杆菌、费尔斯肠杆菌、微小肠杆菌、贝氏肠杆菌D、EbpC和IV型，以及十二指肠球虫组合C。中国污水中还含有猫或狗携带的犬弧菌、微小弧菌、泛型弧菌和毕氏艾美耳球虫D、EbpC和IV型。这一发现表明，宠物动物可能是人类隐孢子虫病、微孢子虫病和贾第鞭毛虫病的宿主，也是中国水污染的潜在来源。(C)2015爱思唯尔B.V.保留所有权利。
This study investigated 319 fecal specimens of cats (n=52) and dogs (n =267) from Heilongjiang province, China for the prevalence and genetic characteristics of Cryptosporidium, Enterocytozoon bieneusi, and Giardia duodenalis. PCR and DNA sequence analysis of the small subunit rRNA gene identified C. felis and C parvum in one cat each (3.8%) and C. canis and C ubiquitum in 6 dogs (2.2%). Polymorphisms in the ribosomal internal transcribed spacer and phylogenetic analysis characterized zoonotic E. bieneusi genotypes D, EbpC, NED1, and NED2 and host-adapted ones NED3, NED4, and PtEb IX in 18 dogs (6.7%) and human-pathogenic genotypes D and IV in 3 cats (5.8%). Genotyping based on the hypermutation of G. duodenalis triosephosphate isomerase gene (TPI) facilitated identification of assemblage F in a cat (1.9%) and assemblages C and E in 12 dogs (4.5%). Subtypes of G. duodenalis isolates were determined by measuring the diversity of both TPI nucleotide and amino acid sequences. C canis, C. fells, C parvum, E. bieneusi genotypes D, EbpC, and IV, and G. duodenalis assemblage C identified herein have been documented in human infections in China. C. canis, C parvum, C. ubiquitum, and E. bieneusi genotypes D, EbpC, and IV carried by cats or dogs also existed in wastewater in China. The finding suggested pet animals could be reservoirs for human cryptosporidiosis, microsporidiosis, and giardiasis and potential sources of water contamination in China. (C) 2015 Elsevier B.V. All rights reserved.