Antiviral Activity of Daphnoretin Isolated from Wikstroemia indica
Antiviral Activity of Daphnoretin Isolated from Wikstroemia indica
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DOI：
10.1002/ptr.2935
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发表时间：
2010-05-01
影响因子：
7.2
通讯作者：
Li, Yao-Lan
中科院分区：
文献类型：
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作者：
Ho, Wing-Shan;Xue, Jun-Yi;Li, Yao-Lan
采用细胞病变效应（CPE）还原试验，对不同极性有机溶剂提取的挥发油进行了抗呼吸道合胞病毒（RSV）活性筛选。乙酸乙酯级分对RSV的活性最高，50%抑制浓度（IC 50）值< 3.9 μ g/mL，选择性指数（SI）> 64.1。进一步分离和纯化该级分得到纯化的化合物，瑞香素。使用空斑减少测定法测试了瑞香素的抗RSV活性，发现其对RSV具有活性，IC 50值为5.87 μ g/mL，SI值为28.17。抗病毒作用方式研究表明，瑞香素对病毒感染的早期事件有轻微的抑制作用，但其作用主要是在复制周期的后期。版权所有（C）2009约翰威利父子有限公司
The ethanol extract of Wikstroemia indica was fractionated with organic solvents of different polarities, and various fractions were screened for their antiviral activity against respiratory syncytial virus (RSV) using a cytopathic effect (CPE) reduction assay. The ethyl acetate fraction was most active against RSV with 50% inhibition concentration (IC50) value < 3.9 mu g/mL and a selectivity index (SI) > 64.1. Further isolation and purification of the fraction led to a purified compound, daphnoretin. Daphnoretin was tested for its anti-RSV activity using a plaque reduction assay and found active against RSV, with an IC50 value of 5.87 mu g/mL and SI value of 28.17. The mode of antiviral action study revealed that daphnoretin could slightly inhibit the early events of the viral infection but its effect was mainly on the later phase of the replication cycle. Copyright (C) 2009 John Wiley & Sons, Ltd.