Identification of candidate signaling genes including regulators of chromosome condensation 1 protein family differentially expressed in the soybean–Phytophthora sojae interaction
Identification of candidate signaling genes including regulators of chromosome condensation 1 protein family differentially expressed in the soybean–Phytophthora sojae interaction
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DOI：
10.1007/s00122-008-0895-z
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发表时间：
2009-02
影响因子：
5.4
通讯作者：
N. Narayanan;Sehiza Grosic;I. M. Tasma;D. Grant;R. Shoemaker;M. Bhattacharyya
中科院分区：
文献类型：
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作者：
N. Narayanan;Sehiza Grosic;I. M. Tasma;D. Grant;R. Shoemaker;M. Bhattacharyya
大豆根腐病是由大豆疫霉（Phytophthora sojae）卵菌引起的一种严重的大豆病害。在大豆品种中利用抗疫基因（Rps）已有效地控制了这一病害。抗病基因编码的抗性通过激活防御反应来表现。为了筛选大豆抗疫基因表达的候选信号基因，以抗疫大豆品种的下胚轴组织为材料，构建了抗疫大豆下胚轴组织的cDNA文库。来自该文库的4，737个表达序列标签（EST）的源自未胁迫cDNA文库的101，833个表达序列标签（EST）的计算机减法导致鉴定出未胁迫文库中不存在的204个基因。在204个已鉴定的基因中，有7个是P. sojaegenes。基于序列比较，204个基因中的91个的推定功能不能被分配。通过对204个基因的宏芯片分析，共鉴定出60个基因，其中包括15个信号转导相关基因和3个P. sojaegenes，其转录本在P.大豆感染的组织中与水对照相比。8个大豆基因在P.与水对照相比，通过进行北方和RT-PCR分析证实了一些选定基因的差异表达。我们已经表明，两个假定的调节染色体凝聚1（RCC 1）家族蛋白下调不相容的相互作用。这一观察结果表明，核质运输蛋白和非编码RNA的功能受到抑制表达的种族特异性phytophthoraresistance。从几乎立即收获的组织中产生的cDNA文库的表征P.大豆感染抗病品种使我们能够鉴定出许多可能参与调节防御相关途径表达的候选信号基因，以表达大豆中的抗疫性。
Stem and root rot caused by the oomycete pathogen,Phytophthora sojae,is a serious soybean disease. Use ofPhytophthoraresistance genes (Rps) in soybean cultivars has been very effective in controlling this pathogen. Resistance encoded byRpsgenes is manifested through activation of defense responses. In order to identify candidate signaling genes involved in the expression ofPhytophthoraresistance in soybean, a cDNA library was prepared from infected etiolated hypocotyl tissues of aPhytophthoraresistant soybean cultivar harvested 2 and 4 h followingP. sojaeinoculation.In silicosubtraction of 101,833 expressed sequence tags (ESTs) originating from unstressed cDNA libraries from 4,737 ESTs of this library resulted in identification of 204 genes that were absent in the unstressed libraries. Of the 204 identified genes, seven wereP. sojaegenes. Putative function of 91 of the 204 genes could not be assigned based on sequence comparison. Macroarray analyses of all 204 genes led to identification of 60 genes including 15 signaling-related soybean genes and threeP. sojaegenes, transcripts of which were induced twofold inP. sojae-infected tissues as compared to that in water controls. Eight soybean genes were down-regulated twofold followingP. sojaeinfection as compared to water controls. Differential expression of a few selected genes was confirmed by conducting Northern and RT-PCR analyses. We have shown that two putative regulators of chromosome condensation 1 (RCC1) family proteins were down-regulated in the incompatible interaction. This observation suggested that the nucleocytoplasmic transport function for trafficking protein and non-coding RNA is suppressed during expression of race-specificPhytophthoraresistance. Characterization of a cDNA library generated from tissues harvested almost immediately followingP. sojae-infection of a resistant cultivar allowed us to identify many candidate signaling genes that are presumably involved in regulating the expression of defense-related pathways for expression ofPhytophthoraresistance in soybean.