Identification of novel stress-responsive biomarkers from gene expression datasets in tomato roots.
Identification of novel stress-responsive biomarkers from gene expression datasets in tomato roots.
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DOI：
10.1071/fp15385
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发表时间：
2016-08
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通讯作者：
Almudena Ferrández-Ayela;A. Sánchez-García;C. Martínez-Andújar;Z. Kevei;M. Gifford;A. Thompson;F. Pérez-Alfocea;J. Pérez-Pérez-J.-Pérez-Pérez-1398026949
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作者：
Almudena Ferrández-Ayela;A. Sánchez-García;C. Martínez-Andújar;Z. Kevei;M. Gifford;A. Thompson;F. Pérez-Alfocea;J. Pérez-Pérez-J.-Pérez-Pérez-1398026949
非生物胁迫，如热，干旱或盐度已被广泛研究。然而，在自然界和田间，植物和作物通常暴露于不同的胁迫组合，这通常导致由几种分子途径的激活介导的协同反应，这不能从对每种单独胁迫的反应中推断出来。通过筛选不同植物种类和不同胁迫下的基因芯片数据，我们鉴定了几个保守的胁迫响应基因，它们在番茄（Solanum lycopersicum L.）根对一种或几种压力的反应。我们验证了这些基因中的10个作为可靠的生物标志物，其表达水平与适应性应激反应中涉及的不同信号通路相关。此外，在这项工作中确定的基因可以被用作一般的盐胁迫生物标志物，以快速评估耐盐品种和野生物种的反应，其中足够的遗传信息还没有。
Abiotic stresses such as heat, drought or salinity have been widely studied individually. Nevertheless, in the nature and in the field, plants and crops are commonly exposed to a different combination of stresses, which often result in a synergistic response mediated by the activation of several molecular pathways that cannot be inferred from the response to each individual stress. By screening microarray data obtained from different plant species and under different stresses, we identified several conserved stress-responsive genes whose expression was differentially regulated in tomato (Solanum lycopersicum L.) roots in response to one or several stresses. We validated 10 of these genes as reliable biomarkers whose expression levels are related to different signalling pathways involved in adaptive stress responses. In addition, the genes identified in this work could be used as general salt-stress biomarkers to rapidly evaluate the response of salt-tolerant cultivars and wild species for which sufficient genetic information is not yet available.