Tyrosine phosphorylated proteins decrease during differentiation of neuronal and glial cells.
Tyrosine phosphorylated proteins decrease during differentiation of neuronal and glial cells.
复制标题
酪氨酸磷酸化蛋白在神经元和神经胶质细胞分化过程中减少。
DOI：
10.1159/000111856
复制
发表时间：
1990
影响因子：
2.9
通讯作者：
Maness,PF
中科院分区：
文献类型：
--
作者：
Ingraham,CA;Maness,PF
酪氨酸激酶与神经系统的发育有关。为了研究它们的作用，使用磷酸酪氨酸抗体进行免疫印迹来鉴定大鼠和小鼠神经胶质和神经元分化过程中酪氨酸激酶的底物。在少突胶质细胞 2 型星形胶质细胞 (02A) 祖细胞中检测到 14 种重要的含磷酸酪氨酸的蛋白质。当 02A 细胞分化为 2 型星形胶质细胞时，74 kDa 的磷酸酪氨酸修饰蛋白的丰度下降了 15 倍，而 36-40 kDa 的含磷酸酪氨酸的蛋白的丰度也下降了。当 02A 细胞分化为少突胶质细胞时，明显的 71 kDa 磷酸酪氨酸修饰蛋白变得不可检测。在视黄酸诱导小鼠胚胎癌细胞系 P19S101A1 (P19) 的神经元分化过程中，80 kDa 的含磷酸酪氨酸的蛋白质在聚集 96 小时后从未分化状态的高水平降至不可检测的水平。视黄酸处理还导致 46 kDa 磷酸酪氨酸蛋白水平迅速下降。由于聚集而发生的细胞与细胞接触导致视黄酸诱导培养物和对照培养物中含 130 kDa 和 90 kDa 磷酸酪氨酸的蛋白质减少。培养的大鼠中枢神经系统大脑皮层神经元和周围神经系统背根神经节神经元表现出明显的 90 和 46 kDa 磷酸酪氨酸修饰蛋白，其大小与 P19 神经元中的蛋白相同。视黄酸诱导的 P19 细胞向神经元分化过程中涉及的磷酸酪氨酸蛋白与 02A 细胞向星形胶质细胞或少突胶质细胞的胶质细胞分化过程中所改变的蛋白不同，表明神经系统分化过程中修饰的酪氨酸激酶底物可能具有细胞类型特异性。
Tyrosine kinases have been implicated in the development of the nervous system. To investigate their role, immunoblotting with phosphotyrosine antibodies was used to identify substrates of tyrosine kinases during glial and neuronal differentiation in the rat and mouse. Fourteen prominent phosphotyrosine-containing proteins were detected in oligodendrocyte-type 2 astrocyte (02A) progenitor cells. When 02A cells differentiated into type 2 astrocytes, a phosphotyrosine-modified protein of 74 kilodaltons (kDa) decreased 15-fold in abundance, and phosphotyrosine-containing proteins of 36–40 kDa declined. When 02A cells differentiated into oligodendrocytes, a prominent 71-kDa phosphotyrosine-modified protein became undetectable. During retinoic acid-induced neuronal differentiation of the mouse embryonal carcinoma cell line P19S101A1 (P19), an 80-kDa phosphotyrosine-containing protein decreased from high levels in the undifferentiated state to undetectable levels after 96 h in aggregation. Retinoic acid treatment also caused a rapid decrease in levels of a 46-kDa phosphotyrosine-containing protein. Cell-cell contact occurring as a result of aggregation resulted in decreases in 130- and 90-kDa phosphotyrosine-containing proteins in both retinoic acid-induced and control cultures. Cultured rat central nervous system cerebral cortical neurons and peripheral nervous system dorsal root ganglion neurons exhibited prominent phosphotyrosine-modified proteins of 90 and 46 kDa the same sizes as those in P19 neurons. The phosphotyrosine-containing proteins involved in the retinoic acid-induced differentiation of P19 cells to neurons were different from those altered in the glial differentiation of 02A cells to astrocytes or oligodendrocytes, indicating that the tyrosine kinase substrates modified during nervous system differentiation may be cell-type-specific.