Prevalence and characteristics of Plasmodium vivax Gametocytes in Duffy-positive and Duffy-negative populations across Ethiopia.
Prevalence and characteristics of Plasmodium vivax Gametocytes in Duffy-positive and Duffy-negative populations across Ethiopia.
复制标题
埃塞俄比亚达菲阳性和达菲阴性人群中间日疟原虫配子细胞的患病率和特征。
DOI：
10.1101/2023.12.10.23299780
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发表时间：
2023
期刊：
影响因子：
--
通讯作者：
Lo,Eugenia
中科院分区：
文献类型：
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作者：
Little,Ebony;Shenkutie,TassewT;Negash,MesheshaTsigie;Abagero,BekaR;Abebe,Abnet;Popovici,Jean;Mekasha,Sindew;Lo,Eugenia
疟原虫在人类宿主体内无性繁殖。携带配子体的感染比例是人与蚊子传播率的指标。尚不清楚达菲阴性的间日疟原虫感染中有多大比例携带配子体。这项研究的目的是确定间日疟原虫在埃塞俄比亚广大地区达菲阴性患者中的流行率，并确定寄生虫的阶段。采集指刺血样进行镜检和分子筛查疟原虫和个体Duffy状态。用SYBR Green和Taqman定量聚合酶链式反应方法进行疟原虫种的分子筛选和Duffy血型基因分型。447份标本中，间日疟原虫确诊414份(92.6%)，达菲阴性16份(3.9%)。其中5/16(31.3%)间日疟原虫Duffy阴性感染标本的配子体阳性。在398例Duffy阳性间日疟原虫感染标本中，150例(37.7%)检出配子体，略高于Duffy阴性标本。这项研究强调了间日疟原虫配子体在达菲阴性感染中的存在，提示人与蚊子之间的传播性。虽然Duffy阴性的间日疟原虫感染通常与低寄生虫血症有关，但其中一些感染表现为相对较高的寄生虫血症，可能代表了间日疟原虫较好的红细胞入侵能力和有助于传播的隐蔽宿主。更好地了解间日疟原虫的传播生物学和配子体功能，特别是在达菲阴性人群中，将有助于非洲未来间日疟的治疗和管理。
Plasmodium parasites replicate asexually in the human host. The proportion of infections that carries gametocytes is a proxy for human-to-mosquito transmissibility. It is unclear what proportion of P. vivax infections in Duffy-negatives carries gametocytes. This study aims to determine the prevalence of P. vivax in Duffy-negatives across broad regions of Ethiopia and characterize parasite stages. Finger-prick blood samples were collected for microscopic and molecular screening of Plasmodium parasites and Duffy status of individuals. Molecular screening of plasmodium species and Duffy blood group genotyping was done using SYBR green and Taqman qPCR method. Among the total 447 samples, 414 (92.6%) were P. vivax confirmed and, 16 (3.9%) of them were from Duffy-negatives. Of these, 5/16 (31.3%) Duffy-negative P. vivax-infected samples were detected with gametocytes. Of the 398 Duffy-positive P. vivax-infected samples, 150 (37.7%) were detected with gametocytes, slightly higher than that in Duffy-negatives. This study highlights the presence of P. vivax gametocytes in Duffy-negative infections, suggestive of human-to-mosquito transmissibility. Although P. vivax infections in Duffy-negatives are commonly associated with low parasitemia, some of these infections were shown with relatively high parasitemia and may represent better erythrocyte invasion capability of P. vivax and hidden reservoirs that can contribute to transmission. A better understanding of P. vivax transmission biology and gametocyte function particularly in Duffy-negative populations would aid future treatment and management of vivax malaria in Africa