PENETRATION OF SEMLIKI FOREST VIRUS FROM ACIDIC PRELYSOSOMAL VACUOLES
PENETRATION OF SEMLIKI FOREST VIRUS FROM ACIDIC PRELYSOSOMAL VACUOLES
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DOI：
10.1016/0092-8674(83)90078-8
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发表时间：
1983-01-01
期刊：
影响因子：
64.5
通讯作者：
HELENIUS, A
中科院分区：
文献类型：
--
作者：
MARSH, M;BOLZAU, E;HELENIUS, A
为了鉴定和表征塞姆利基森林病毒（SFV）渗透到宿主细胞胞质区室的细胞内位点，测定了仓鼠肾BHK-21细胞中核衣壳脱壳和感染的时间过程和温度依赖性。在37度。在病毒内吞作用后5-7分钟内检测到基因组释放到胞质溶胶中，病毒颗粒递送到次级溶酶体发生在15-20分钟内。20.degree. C病毒颗粒通过内吞作用被内化，但它们不被递送到次级溶酶体。在20度。C核衣壳脱壳和感染发生，表明次级溶酶体是不需要SFV的渗透。因此，穿透反应显然发生在前溶酶体内吞空泡（内体）中。由于通过膜融合的SFV渗透需要pH < 6并且在靶膜中存在胆固醇，因此数据表明内体是酸性的并且含有胆固醇。
To identify and characterize the intracellular site from which the penetration of Semliki Forest virus (SFV) to the cytosolic compartment of the host cell occurs, the time course and temperature dependence of nucleocapsid uncoating and infection in hamster kidney BHK-21 cells was determined. At 37.degree. C the genome release to the cytosol was detected within 5-7 min after virus endocytosis, delivery of the virus particles to secondary lysosomes occurred within 15-20 min. At temperatures of 15.degree.-20.degree. C virus particles were internalized by endocytosis, but they were not delivered to the secondary lysosomes. At 20.degree. C nucleocapsid uncoating and infection occurred, indicating that secondary lysosomes are not required for SFV penetration. Thus, the penetration reaction apparently occurs in prelysosomal endocytic vacuoles (endosomes). As SFV penetration by membrane fusion requires a pH < 6 and the presence of cholesterol in the target membrane, the data indicate that endosomes are acidic and contain cholesterol.