Fengycin Produced by Bacillus amyloliquefaciens FZB42 Inhibits Fusarium graminearum Growth and Mycotoxins Biosynthesis
Fengycin Produced by Bacillus amyloliquefaciens FZB42 Inhibits Fusarium graminearum Growth and Mycotoxins Biosynthesis
复制标题
解淀粉芽孢杆菌 FZB42 产生的风霉素抑制禾谷镰刀菌生长和霉菌毒素生物合成
DOI：
10.3390/toxins11050295
复制
发表时间：
2019-05-01
期刊：
影响因子：
4.2
通讯作者：
Gao, Xuewen
中科院分区：
文献类型：
--
作者：
Hanif, Alvina;Zhang, Feng;Gao, Xuewen
禾谷镰刀菌是一种引起禾谷类作物赤霉病的病原菌。它会产生次生代谢物，如脱氧雪腐镰刀菌烯醇，降低谷物质量，导致作物减产。已经开发了许多策略来防治这种病原真菌；然而，考虑到缺乏抗性品种和使用化学农药可能造成的环境危害，人们的努力转向了植物病害的生物防治，这是一种可持续和生态友好的方法。从解淀粉芽孢杆菌FZB42粗提物中分离得到一种分离纯化的蜂霉素，并用MALDI-TOF-MS对其进行了分析。通过扫描电子显微镜观察到，它的应用导致真菌菌丝的结构变形。在植物试验中，揭示了风霉素对禾谷镰刀菌生长的抑制作用，并突出了其抗病能力。风霉素显著抑制了小麦赤霉病的侵染，并降低了脱氧雪腐镰刀菌烯醇(DON)、3-乙酰脱氧雪腐镰刀菌烯醇(3-AdOn)、15-乙酰脱氧雪腐镰刀菌烯醇(15-AdOn)和玉米赤霉烯酮(Zen)的产量。综上所述，我们报道了淀粉溶解芽孢杆菌FZB42产生的风霉素具有作为禾谷镰刀菌生防剂的潜力，并能抑制该真菌产生的真菌毒素。
Fusarium graminearum is a notorious pathogen that causes Fusarium head blight (FHB) in cereal crops. It produces secondary metabolites, such as deoxynivalenol, diminishing grain quality and leading to lesser crop yield. Many strategies have been developed to combat this pathogenic fungus; however, considering the lack of resistant cultivars and likelihood of environmental hazards upon using chemical pesticides, efforts have shifted toward the biocontrol of plant diseases, which is a sustainable and eco-friendly approach. Fengycin, derived from Bacillus amyloliquefaciens FZB42, was purified from the crude extract by HPLC and further analyzed by MALDI-TOF-MS. Its application resulted in structural deformations in fungal hyphae, as observed via scanning electron microscopy. In planta experiment revealed the ability of fengycin to suppress F. graminearum growth and highlighted its capacity to combat disease incidence. Fengycin significantly suppressed F. graminearum, and also reduced the deoxynivalenol (DON), 3-acetyldeoxynivalenol (3-ADON), 15-acetyldeoxynivalenol (15-ADON), and zearalenone (ZEN) production in infected grains. To conclude, we report that fengycin produced by B. amyloliquefaciens FZB42 has potential as a biocontrol agent against F. graminearum and can also inhibit the mycotoxins produced by this fungus.