Genome-wide gene amplification during differentiation of neural progenitor cells in vitro.
Genome-wide gene amplification during differentiation of neural progenitor cells in vitro.
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DOI：
10.1371/journal.pone.0037422
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Meese E
中科院分区：
文献类型：
--
作者：
Fischer U;Keller A;Voss M;Backes C;Welter C;Meese E
DNA序列扩增是一种在某些生物体正常发育过程中可预见地发生在特定阶段的现象。发育基因扩增首先在两栖动物的配子发生过程中被描述，但尚未在人类中被描述。迄今为止，人类的基因扩增是许多肿瘤的标志。我们使用阵列CGH（比较基因组杂交）和FISH（荧光原位杂交）发现基因扩增在体外分化的人神经祖细胞。在这里，我们报告了人类神经祖细胞诱导分化两天和五天后复杂的基因扩增模式。我们在神经祖细胞中鉴定了几个已知在恶性肿瘤中扩增的扩增基因。在分化中的神经祖细胞和来源于星形胶质细胞的恶性肿瘤细胞之间，扩增的染色体区域也有显著的重叠。在正常人类细胞中作为生理过程的基因扩增尚未报道，并且可能与两栖动物和昆虫中的发育基因扩增相似。
DNA sequence amplification is a phenomenon that occurs predictably at defined stages during normal development in some organisms. Developmental gene amplification was first described in amphibians during gametogenesis and has not yet been described in humans. To date gene amplification in humans is a hallmark of many tumors. We used array-CGH (comparative genomic hybridization) and FISH (fluorescence in situ hybridization) to discover gene amplifications during in vitro differentiation of human neural progenitor cells. Here we report a complex gene amplification pattern two and five days after induction of differentiation of human neural progenitor cells. We identified several amplified genes in neural progenitor cells that are known to be amplified in malignant tumors. There is also a striking overlap of amplified chromosomal regions between differentiating neural progenitor cells and malignant tumor cells derived from astrocytes. Gene amplifications in normal human cells as physiological process has not been reported yet and may bear resemblance to developmental gene amplifications in amphibians and insects.
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使用人神经元对自闭症的功能基因组学进行建模。
影响因子：
11
作者：
Konopka G;Wexler E;Rosen E;Mukamel Z;Osborn GE;Chen L;Lu D;Gao F;Gao K;Lowe JK;Geschwind DH
通讯作者：
Geschwind DH
影响因子：
10.4
作者：
Moors M;Rockel TD;Abel J;Cline JE;Gassmann K;Schreiber T;Schuwald J;Weinmann N;Fritsche E
通讯作者：
Fritsche E
影响因子：
3
作者：
Svendsen, CN;ter Borg, MG;Caldwell, MA
通讯作者：
Caldwell, MA
DOI：
10.1523/jneurosci.0181-11.2011
发表时间：
2011-08-10
期刊：
The Journal of neuroscience : the official journal of the Society for Neuroscience
影响因子：
--
作者：
Mukamel Z;Konopka G;Wexler E;Osborn GE;Dong H;Bergman MY;Levitt P;Geschwind DH
通讯作者：
Geschwind DH
DOI：
10.1126/science.1164382
发表时间：
2008-09-26
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Parsons DW;Jones S;Zhang X;Lin JC;Leary RJ;Angenendt P;Mankoo P;Carter H;Siu IM;Gallia GL;Olivi A;McLendon R;Rasheed BA;Keir S;Nikolskaya T;Nikolsky Y;Busam DA;Tekleab H;Diaz LA Jr;Hartigan J;Smith DR;Strausberg RL;Marie SK;Shinjo SM;Yan H;Riggins GJ;Bigner DD;Karchin R;Papadopoulos N;Parmigiani G;Vogelstein B;Velculescu VE;Kinzler KW
通讯作者：
Kinzler KW