Cytokine regulation of fibroblast growth factor receptor 3 IIIb in intestinal epithelial cells
Cytokine regulation of fibroblast growth factor receptor 3 IIIb in intestinal epithelial cells
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DOI：
10.1152/ajpgi.1997.272.4.g885
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发表时间：
1997-04-01
影响因子：
4.5
通讯作者：
Podolsky, DK
中科院分区：
文献类型：
--
作者：
Kanai, M;Rosenberg, I;Podolsky, DK
肠上皮细胞的增殖和功能由一系列调节肽调节，包括细胞因子和肽生长因子。为了确定整合这些调节系统的机制，在Caco-2细胞中检查了生长因子和细胞因子对肠上皮细胞上表达的成纤维细胞生长因子（FGF）受体3（FGFRS）IIIb的表达的影响。FGFRS IIIb的调节表达与分化状态的获得相关。角质细胞生长因子（KGF），FGF受体家族的另一个成员的配体，增强FGFR 3 IIIb的表达，但酸性FGF，FGFR 3 IIIb本身的配体，没有影响。表皮生长因子和转化生长因子-β也在不同的时间模式中显著增强FGFR 3 IIIb表达。此外，FGFRS IIIb表达通过细胞因子白细胞介素-2增加10倍。这些研究证明了细胞因子和生长因子配体-受体系统在肠上皮细胞中的整合。
Proliferation and function of the intestinal epithelium is modulated by a range of regulatory peptides, including cytokines and peptide growth factors. To define mechanisms integrating these regulatory systems, the effects of growth factors and cytokines on the expression of the fibroblast growth factor (FGF) receptor 3 (FGFRS) IIIb expressed on intestinal epithelial cells were examined in Caco-2 cells. Regulated expression of FGFRS IIIb was associated with acquisition of the differentiated state. Keratinocyte growth factor (KGF), a ligand of another member of the FGF receptor family, enhanced expression of FGFR3 IIIb, but acidic FGF, the ligand for FGFR3 IIIb itself, had no effect. Epidermal growth factor and transforming growth factor-beta also markedly enhanced FGFR3 IIIb expression in a different temporal pattern. In addition, FGFRS IIIb expression was increased 10-fold by the cytokine interleukin-2. These studies demonstrate integration between cytokines and growth factor ligand-receptor systems in intestinal epithelial cells.