The effects of equal caloric high fat and western diet on metabolic syndrome, oxidative stress and vascular endothelial function in mice
The effects of equal caloric high fat and western diet on metabolic syndrome, oxidative stress and vascular endothelial function in mice
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DOI：
10.1111/apha.12253
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发表时间：
2014-07-01
影响因子：
6.3
通讯作者：
Savontaus, E.
中科院分区：
文献类型：
--
作者：
Heinonen, I.;Rinne, P.;Savontaus, E.
目的营养有助于肥胖的增加，但高脂肪饮食而不是西式饮食是否会加剧肥胖的发展以及代谢综合征和血管功能的其他特征仍有待确定。方法采用雄性C57B1/6N小鼠，观察高脂(45%大卡)饮食(HFD)和等热量西方饮食(WD)对高脂、高糖、高胆固醇饮食8周的影响。结果高脂饮食组和低脂饮食组小鼠的体脂含量(体脂百分比)显著高于低脂饮食对照组(LFD)。然而，与其他组相比，只有HFD组小鼠的总体重更高。WD组小鼠肝脏甘油三酯、胆固醇和氧化损伤的含量高于LFD组小鼠。两组间空腹血糖或血清胰岛素、血清或肌肉甘油三酯、糖耐量或收缩压无显著差异，但血清游离脂肪酸较LFD组升高。作为氧化应激标志的组织和血清双烯结合水平的增加是明显的，尤其是在WD小鼠中。HFD小鼠肠系膜小动脉的内皮依赖性舒张功能显著受损，但在主动脉中未见明显损害。在WD组，最大松弛与身体肥胖呈负相关，而在HFD小鼠中则不相关。结论在不改变体重的情况下，西餐可引起明显的全身氧化应激，增加体脂，这与阻力动脉的内皮功能有关。
Aim Nutrition contributes to increased adiposity, but it remains to be determined whether high fat rather than Western diet exacerbates the development of obesity and other characteristics of metabolic syndrome and vascular function. Methods We studied the effects of high fat (45% kcal) diet (HFD) and equal caloric Western diet (WD) high in fat, sucrose and cholesterol for 8weeks in male C57B1/6N mice. Results Mice fed with HFD and WD showed substantially higher body adiposity (body fat %) compared with control mice receiving low fat (10%) diet (LFD). However, total body weight was higher only in HFD mice compared with other groups. The amount of liver triglycerides, cholesterol and oxidative damage was higher in WD mice compared with mice on LFD. There were no significant differences in fasting blood glucose or serum insulin, serum or muscle triglycerides, glucose tolerance or systolic blood pressure between the groups, but serum free fatty acids were increased in HFD mice compared with LFD. Increased levels of tissue and serum diene conjugation as a marker of oxidative stress were evident especially in WD mice. The endothelium-dependent relaxations were significantly impaired in the small mesenteric arteries of HFD mice, but not in the aorta. Maximal relaxations correlated negatively with body adiposity in WD but not in HFD mice. Conclusions The major finding in the present study is that without changing body weight, Western diet induces marked whole-body oxidative stress and elevates body adiposity, which associates with the endothelial function of resistance arteries.