The Formation of Polyploid Cells in Ripening Cotyledons of Pisum sativum L. in Relation to Ribosome and Protein Synthesis
The Formation of Polyploid Cells in Ripening Cotyledons of Pisum sativum L. in Relation to Ribosome and Protein Synthesis
复制标题
豌豆成熟子叶多倍体细胞的形成与核糖体和蛋白质合成的关系
DOI：
10.1093/jxb/24.1.216
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发表时间：
1973
影响因子：
6.9
通讯作者：
R. Parijs
中科院分区：
文献类型：
--
作者：
A. Scharpé;R. Parijs
本文研究了豌豆子叶发育过程中薄壁细胞多倍体核的形成及RNA和蛋白质的合成。在种子形成过程中，这些细胞合成大量的储备蛋白质和淀粉，这些蛋白质和淀粉随后在种子萌发过程中被胚消耗。用化学方法测定了整个子叶中DNA、RNA和蛋白质的含量。在准备阶段，在真实的细胞扩展和种子球蛋白和淀粉的密集积累阶段之前，贮藏细胞达到高水平的多倍性：结果表明，子叶薄壁细胞的高度多倍性与RNA和蛋白质的高合成速率有关（种子球蛋白）在这个存储器官（基因剂量效应）。
The present paper deals with the formation of polyploid nuclei and the synthesis of RNA and protein in the parenchyma cells of developing cotyledons ofPisum sativumL. During seed formation these cells synthesize large amounts of reserve proteins and starch, which later on are used up by the embryo during seed germination.The changes of the amount of DNA per cell in the ripening cotyledon tissue have been estimated by Feulgen histophotometry. The amounts of DNA, RNA, and protein in the whole cotyledons have been estimated by chemical methods. In this way it was possible to correlate the fluctuations of the amount of DNA, RNA, and protein with changes at the cellular level.During a preparatory phase, preceding the phase of real cell expansion and intensive accumulation of seed globulins and starch, the storage cells attain a high level of polyploidy: nuclei with up to a 64 C DNA content are formed.The results indicate a correlation between the high degree of polyploidy in the parenchyma cells of the cotyledon and the high rate of RNA and protein synthesis (seed globulins) in this storage organ (gene dosage effect).