Fabrication and characterization of complex nanoparticles based on carboxymethyl short chain amylose and chitosan by ionic gelation
Fabrication and characterization of complex nanoparticles based on carboxymethyl short chain amylose and chitosan by ionic gelation
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离子凝胶化基于羧甲基短链直链淀粉和壳聚糖的复合纳米粒子的制备和表征
DOI：
10.1039/c8fo00238j
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发表时间：
2018-05-01
期刊：
影响因子：
6.1
通讯作者：
Li, Caiming
中科院分区：
文献类型：
--
作者：
Ji, Na;Hong, Yan;Li, Caiming
我们的目的是研究短链直链淀粉(SCA)与壳聚糖(CS)通过静电相互作用修饰后是否可以作为口服生物活性成分的候选药物。以羧甲基短链直链淀粉(CMSCA)为原料，与一氯乙酸反应合成了羧甲基短链直链淀粉(CMSCA)。用Zeta电位测定、傅立叶红外光谱、差示扫描量热法、热重法和导数热重法研究了反应后SCA含量的变化。以羧甲基壳聚糖和壳聚糖为原料，采用离子凝胶法合成了复合纳米粒子。FTIR光谱分析表明，复合纳米粒子是通过CMSCA与CS之间的氢键和静电相互作用合成的。CMSCA/CS纳米粒对胰岛素的包封率为85.2%，体外具有较好的缓释作用。细胞培养表明CMSCA/CS纳米粒具有良好的细胞相容性。这些结果表明，离子凝胶法构建的CMSCA/CS纳米粒可以进一步开发为一种潜在的多肽药物口服给药系统。
We aimed to investigate whether the combination of the modification of short chain amylose (SCA) with chitosan (CS) through the electrostatic interaction could be considered as a candidate for oral delivery of bioactive ingredients. Carboxymethyl short chain amylose (CMSCA) was synthesized by reacting SCA with monochloroacetic acid. The changes in SCA levels after the reaction were investigated by zeta-potential determination, Fourier transform infrared (FTIR) spectroscopy, differential scanning calorimetry, thermogravimetry and derivative thermogravimetry. Complex nanoparticles (NPs) were then synthesized using CMSCA and CS by ionic gelation. FTIR spectral analysis revealed that the complex NPs were synthesized by hydrogen bonding and electrostatic interactions between CMSCA and CS. CMSCA/CS NPs show an insulin encapsulation efficiency of 85.2% and exhibit sustained release of insulin in vitro. CMSCA/CS NPs were observed to show excellent cytocompatibility by cell culture. These findings demonstrated that CMSCA/CS NPs constructed by the ionic gelation method could be further exploited as a potential oral delivery system for peptide drugs.