Gene silencing results in instability of antibody production in transgenic plants
Gene silencing results in instability of antibody production in transgenic plants
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DOI：
10.1007/s004380050932
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发表时间：
1999-01-01
期刊：
影响因子：
--
通讯作者：
Depicker, A
中科院分区：
文献类型：
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作者：
De Neve, M;De Buck, S;Depicker, A
在五个不同的纯合转基因拟南芥系中评估抗体和F-ab表达的稳定性。这些品系中的每一个都显示编码抗体多肽的转基因的沉默，导致抗体产生的不稳定性。然而，每条线都有不同的和特定的不稳定性。每个品系中抗体积累水平随发育阶段的特征性变化表明T-DNA整合模式在触发沉默中起作用，并且简单转基因位点的历史和整合位置可以影响表观遗传沉默的易感性。在具有低抗体积累水平的不同品系中，甲基化在单独的启动子中、在启动子和转录区两者中、仅在转录区中、或在转录区和下游序列中被发现。总之，我们的数据表明，表观遗传效应导致不同的转基因表达谱在每一个分析的五个拟南芥品系。
The stability of antibody and F-ab expression was assessed in five different homozygous transgenic Arabidopsis lines. Each of these lines showed silencing of the transgenes that: encode the antibody polypeptides, leading to instability of antibody production. However, each line had a different and specific instability profile. The characteristic variation in the level of antibody accumulation in each line as a function of developmental stage indicated that the T-DNA integration pattern played a role in triggering silencing, and also that the history and the integration position of simple transgene loci can influence the susceptibility to epigenetic silencing. In different lines with low antibody accumulation levels, methylation was found either in the promoter alone, in both the promoter and the transcribed region, in the transcribed region only, or in the transcribed region and downstream sequences. In conclusion, our data suggest that epigenetic effects result in different transgene expression profiles in each of the five Arabidopsis lines analyzed.