MicroProtein: Dynamic and accurate regulation of protein activity
MicroProtein: Dynamic and accurate regulation of protein activity
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MicroProtein：动态准确调节蛋白质活性
DOI：
10.1111/jipb.13229
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发表时间：
2022
期刊：
影响因子：
--
通讯作者：
Shi Hui
中科院分区：
文献类型：
--
作者：
Wu Qingqing;Zhong Shangwei;Shi Hui
蛋白质通常组装低聚物或高阶复合物，以提高其在生物过程中的效率和特异性。复合物形成和破坏的动态平衡对蛋白质功能进行可逆调节。MicroProteins是一种小的单结构域蛋白质，可直接结合靶蛋白复合物并破坏其组装。越来越多的证据表明，microProteins是翻译后水平蛋白质活性的有效调节剂。在过去的几十年里，成千上万的植物microProteins已被预测的计算方法，但只有少数已被实验验证。最近的研究强调了新鉴定的microProteins inhibopsis和其他植物物种的机械工作模式。在这里，我们审查的特点microProteins，包括其生物学作用，调节目标，和行动模式。特别是，我们专注于microProtein-directed allosteric modulation的关键组件在光信号通路，我们总结了已知的microProtein在植物中的生物起源和进化轨迹。了解微蛋白的调控机制是微蛋白在作物生物工程中作为多功能生物技术工具的重要一步。
Proteins usually assemble oligomers or high‐order complexes to increase their efficiency and specificity in biological processes. The dynamic equilibrium of complex formation and disruption imposes reversible regulation of protein function. MicroProteins are small, single‐domain proteins that directly bind target protein complexes and disrupt their assembly. Growing evidence shows that microProteins are efficient regulators of protein activity at the post‐translational level. In the last few decades, thousands of plant microProteins have been predicted by computational approaches, but only a few have been experimentally validated. Recent studies highlighted the mechanistic working modes of newly‐identified microProteins inArabidopsisand other plant species. Here, we review characterized microProteins, including their biological roles, regulatory targets, and modes of action. In particular, we focus on microProtein‐directed allosteric modulation of key components in light signaling pathways, and we summarize the biogenesis and evolutionary trajectory of known microProteins in plants. Understanding the regulatory mechanisms of microProteins is an important step towards potential utilization of microProteins as versatile biotechnological tools in crop bioengineering.