A NEW METHOD FOR IN-VIVO QUANTIFICATION OF CHANGES IN INITIAL ENAMEL CARIES WITH LASER FLUORESCENCE
A NEW METHOD FOR IN-VIVO QUANTIFICATION OF CHANGES IN INITIAL ENAMEL CARIES WITH LASER FLUORESCENCE
复制标题
DOI：
10.1159/000262032
复制
发表时间：
1995-01-01
期刊：
影响因子：
4.2
通讯作者：
ANGMARMANSSON, B
中科院分区：
文献类型：
--
作者：
DEJONG, EDJ;SUNDSTROM, F;ANGMARMANSSON, B
一种新的方法，在体内评估的变化，在初始釉质龋病变的开发和测试。配备有高通滤波器（λ> 520 nm）的CCD照相机收集龋齿的荧光图像，用漫射激光（λ = 488 nm）口内照射。初期病变显示荧光损失，以健康组织的荧光辐射率的百分比表示。PC程序（Inspektor，型号QLF 1.0）用于显示、存储和随后的图像分析。为了能够计算荧光损失，根据与病变邻接的健全组织的辐射度来重建病变部位处的健全组织的荧光。该方法在19个视觉上健全的口腔表面在体内进行了测试。实际和重建辐射率之间的差异为-1.6 +/-（SD）1.1%，面积在8和14 mm（2）之间变化。龋齿定量的可重复性通过在体内测量一个被捕的初始龋齿病变25次来测试。病变面积为0.56 +/- 0.20 mm（2），荧光损失为17.6 +/-0.7%，对应于病变深度为17 +/- 2 μ m。新的定量方法应用于体内龋齿模型的测试，该模型使用牙菌斑积聚支架固定在计划拔牙的前磨牙上。在包围前和去包围后0、2、3和5周在体内记录视频图像。记录了不同时间点之间病变大小和矿物质含量的明显变化。因此，该方法似乎适合于在光滑表面上的自然釉质病变的矿物质变化的体内测量，并可能是有用的临床试验和预防措施的评价。
A new method for the in vivo assessment of changes in initial enamel caries lesions was developed and tested. A CCD camera equipped with a high-pass filter (lambda > 520 nm) collects the fluorescence image of carious teeth, illuminated intraorally with diffuse laser light (lambda = 488 nm). Incipient lesions show a loss in fluorescence to be expressed as a percentage of fluorescence radiance of sound tissue. A PC program (Inspektor, model QLF 1.0) is used for display, storage, and subsequent analysis of images. To enable the calculation of fluorescence loss, the fluorescence of sound tissue at the lesion site is reconstructed from the radiances of sound tissue bordering the lesion. This method was tested on 19 visually sound buccal surfaces in vivo. The differences between actual and reconstructed radiance was -1.6 +/- (SD) 1.1%, over areas varying between 8 and 14 mm(2). The repeatability of the caries quantification was tested by measuring one arrested initial caries lesion 25 times in vivo. The lesion area was 0.56 +/- 0.20 mm(2), and the loss of fluorescence was 17.6 +/- 0.7%, corresponding to a lesion depth of 17 +/- 2 mu m The new quantitative method was applied for the testing of an in vivo caries model using plaque-accumulating brackets on premolars scheduled for extraction. Videoimages were recorded in vivo before bracketing and 0, 2, 3, and 5 weeks after debracketing. Clear changes between the different time points were recorded for both lesion size and mineral content. Thus, the method seems suitable for in vivo measurement of mineral changes in natural enamel lesions on smooth surfaces and might be useful for clinical trials and evaluation of preventive measures.