Electrospray ionization detection of inherently nonresponsive epoxides by peptide binding.
Electrospray ionization detection of inherently nonresponsive epoxides by peptide binding.
复制标题
通过肽结合对固有无响应环氧化物进行电喷雾电离检测。
DOI：
10.1002/rcm.337
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发表时间：
2001
期刊：
影响因子：
--
通讯作者：
Enke,CG
中科院分区：
文献类型：
--
作者：
Cech,NB;Krone,JR;Enke,CG
一种对电喷雾分析固有无响应的小有机分子1,3 -二氧化丁二烯，通过电喷雾电离（ESI）将其与各种肽结合并观察产物的特征质量位移来分析。环氧化物只与序列中含有精氨酸的肽反应，很可能通过碱基催化开环形成共价结合产物。对二氧化丁二烯/肽加合物生成了超过3个数量级的校准曲线。几种其他环氧化合物也与所选的肽（血管紧张素II）反应，并且这些环氧化合物与肽的加合物也被观察到，证明了该方法用于分析小环氧化合物的通用性。本研究证明了分析生物样品中结合肽或蛋白质的环氧化物的可能性。此外，它展示了一个重要的概念，可以应用于电喷雾分析中的其他分析问题：能够将对电喷雾分析无反应的分析物与非常适合该技术的分析物反应，并基于两者之间形成的加合物完成定量。版权所有©2001约翰威利父子有限公司
A small organic molecule that is inherently nonresponsive to electrospray analysis, 1,3‐butadiene diepoxide, was analyzed via electrospray ionization (ESI) by binding it to various peptides and observing the product at the characteristic mass shift. The epoxide reacted only with peptides with arginines in their sequence, most likely through a base‐catalyzed ring opening to form a covalently bound product. A calibration curve linear over 3 orders of magnitude was generated for the butadiene diepoxide/peptide adduct. Several other epoxides were also reacted with the peptide of choice (angiotensin II), and adducts of these epoxides with the peptide were observed as well, demonstrating the versatility of this method for the analysis of small epoxides. This study demonstrates the possibility of assaying epoxides bound to peptides or proteins in biological samples. Furthermore, it demonstrates an important concept that could be applied to other analytical problems in electrospray: the ability to react an analyte that is nonresponsive to electrospray analysis with an analyte well suited for the technique, and accomplish quantitation based on the adduct formed between the two. Copyright © 2001 John Wiley & Sons, Ltd.