Identification and expression analysis of putative mRNA-like non-coding RNA in Drosophila
Identification and expression analysis of putative mRNA-like non-coding RNA in Drosophila
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DOI：
10.1111/j.1365-2443.2005.00910.x
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发表时间：
2005-12-01
期刊：
影响因子：
2.1
通讯作者：
Kageyama, Y
中科院分区：
文献类型：
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作者：
Inagaki, S;Numata, K;Kageyama, Y
哺乳动物 cDNA 测序项目得出的最令人惊讶的结果之一是，数千种 mRNA 样非编码 RNA (ncRNA) 得到表达，并构成至少 10% 的聚 (A)(+) RNA。然而，在大多数情况下，这些 RNA 分子的功能仍不清楚。为了阐明 mRNA 样 ncRNA 的生物学意义，我们通过计算筛选了 11 691 个果蝇全长 cDNA。在消除了可能的蛋白质编码转录本后，136 个被鉴定为 mRNA 样 ncRNA 的有力候选者。尽管大多数这些推定的 ncRNA 遍布果蝇属，但预测的氨基酸序列即使在相关物种中也不保守，这表明这些转录本实际上是非编码 RNA。原位杂交分析显示，其中35个转录本在胚胎发生过程中表达，其中27个仅在特定组织中检测到，包括气管系统、中肠原细胞、内脏中胚层、生殖细胞以及中枢和周围神经系统。这些高度调控的表达模式表明，许多 mRNA 样 ncRNA 在果蝇器官发生和细胞分化的多个步骤中发挥着重要作用。这是第一份关于模型生物体中大多数 mRNA 样 ncRNA 在特定组织和细胞类型中表达的报告。
One of the most surprising results to emerge from mammalian cDNA sequencing projects is that thousands of mRNA-like non-coding RNAs (ncRNAs) are expressed and constitute at least 10% of poly(A)(+) RNAs. In most cases, however, the functions of these RNA molecules remain unclear. To clarify the biological significance of mRNA-like ncRNAs, we computationally screened 11 691 Drosophila melanogaster full-length cDNAs. After eliminating presumable protein-coding transcripts, 136 were identified as strong candidates for mRNA-like ncRNAs. Although most of these putative ncRNAs are found throughout the Drosophila genus, predicted amino acid sequences are not conserved even in related species, suggesting that these transcripts are actually non-coding RNAs. In situ hybridization analyses revealed that 35 of the transcripts are expressed during embryogenesis, of which 27 were detected only in specific tissues including the tracheal system, midgut primordial cells, visceral mesoderm, germ cells and the central and peripheral nervous system. These highly regulated expression patterns suggest that many mRNA-like ncRNAs play important roles in multiple steps of organogenesis and cell differentiation in Drosophila. This is the first report that the majority of mRNA-like ncRNAs in a model organism are expressed in specific tissues and cell types.