Localization of Z-protein in isolated Z-disk sheets of chicken leg muscle.
Localization of Z-protein in isolated Z-disk sheets of chicken leg muscle.
复制标题
Z蛋白在鸡腿肌肉的孤立Z盘片中定位。
DOI：
10.1083/jcb.95.1.85
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发表时间：
1982-10
期刊：
影响因子：
--
通讯作者：
Maruyama K
中科院分区：
文献类型：
--
作者：
Ohashi K;Mikawa T;Maruyama K
用Z-蛋白、结蛋白和α-肌动蛋白的抗血清免疫印迹研究表明，Z-蛋白与结蛋白和α-肌动蛋白有明显的区别。Z-蛋白不是另一种蛋白的蛋白水解物，而是鸡胸肌肌原纤维的固有成分。在这些实验中，完整肌肉的十二烷基硫酸钠提取物首先在聚丙烯酰胺凝胶中进行电泳，然后将蛋白质转移到硝酸纤维素纸上。通过用各自的抗血清应用间接免疫荧光技术，可以检测到薄片上的每一种蛋白质。用这些抗血清进行的免疫荧光显微镜研究表明，Z-蛋白与α-肌动蛋白在分离的Z盘片中具有相同的分布。抗Z蛋白抗血清和抗α-肌动蛋白抗血清染色Z盘内。另一方面，结蛋白的抗血清对分离的Z盘片中的Z盘周进行染色。
Immunoblotting studies with antisera against Z-protein, desmin, and alpha-actinin showed that Z-protein is clearly distinguishable from desmin and alpha-actinin. Z-protein is not a proteolytic product of another protein but is an intrinsic component of chicken breast muscle myofibrils. In these experiments, an SDS extract of intact muscle was first electrophoresed in a polyacrylamide gel, and then proteins were transferred to a nitrocellulose paper sheet. Detection of each protein on the sheet was made possible by the application of the indirect immunofluorescence technique with the respective antiserum. Immunofluorescence microscope studies using these antisera revealed that Z-protein has the same distribution as alpha-actinin in isolated Z- disk sheets. Anti-Z-protein antiserum and anti-alpha-actinin antiserum stained the interior of Z-disks. On the other hand, antiserum against desmin stained the periphery of Z-disks in isolated Z-disk sheets.