Critical periods of basic fibroblast growth factor and brain-derived neurotrophic factor in the development of the chicken cochleovestibular ganglion in vitro.
Critical periods of basic fibroblast growth factor and brain-derived neurotrophic factor in the development of the chicken cochleovestibular ganglion in vitro.
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碱性成纤维细胞生长因子和脑源性神经营养因子在鸡耳蜗前庭神经节体外发育的关键期。
DOI：
10.1006/exnr.1997.6623
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发表时间：
1997
期刊：
影响因子：
--
通讯作者：
Morest,DK
中科院分区：
文献类型：
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作者：
Hossain,WA;Rutledge,A;Morest,DK
脑源性神经营养因子（BDNF）和成纤维细胞生长因子-2（FGF-2）在感觉神经元发育中的时间作用已在模拟正常胚胎内耳发育（正常细胞）的细胞培养物中进行了研究。以前的研究表明，FGF-2在体外的前2天刺激神经节细胞的迁移和分化，但在5天后导致变性，暗示其他因素在其后期的发展。为了观察BDNF是否是这样一个因子，我们从白色来航牛胚胎中分离出耳囊，此时神经节细胞前体细胞通常开始从耳上皮迁移。培养物在含有或不含有人重组FGF-2或含有BDNF的成分确定的培养基中生长2天。在第3天，FGF-2用在成分确定的培养基中的BDNF或仅用成分确定的培养基替换。在活体培养中，通过延时成像测量成神经细胞迁移和神经突生长。在第3天接受BDNF的培养物中，细胞迁移和从外植体的神经突生长增加超过3周，但在第3天仅接受成分确定的培养基的培养物中没有。当从第一天起接受在限定培养基中的BDNF或单独的限定培养基时，培养物存活不超过3 - 4天。BDNF的中和抗体抑制神经元迁移和轴突生长，也阻断了外源性BDNF的作用。BDNF并没有通过与FGF-2相互作用来增强FGF-2的作用。这些实验确定了一个时间顺序，其中FGF-2在发育早期起作用，而BDNF影响后期。
The temporal roles of brain-derived neurotrophic factor (BDNF) and fibroblast growth factor-2 (FGF-2) in the development of sensory neurons have been studied in a cell culture preparation which models normal embryonic inner ear development (normocytic). Previous studies showed that FGF-2 stimulated migration and differentiation of ganglion cells for the first 2 daysin vitro,but after 5 days led to degeneration, implicating other factors in their later development. To see if BDNF could be such a factor, otocysts were explanted from white leghorn embryos at the time when ganglion cell precursors normally start migrating from the otic epithelium. Cultures were grown in a defined medium, either with or without human recombinant FGF-2 for 2 days or with BDNF. On Day 3, FGF-2 was replaced either with BDNF in defined medium or with defined medium only. Measurements of neuroblast migration and neurite outgrowth were made by time-lapse imaging in living cultures. In cultures receiving BDNF on Day 3, cell migration and neurite outgrowth from the explant increased for more than 3 weeks but not in cultures receiving only defined medium from Day 3. Cultures did not survive more than 3–4 days when receiving either BDNF in defined medium or defined medium alone from the first day. A neutralizing antibody to BDNF inhibited neuronal migration and neurite outgrowth, and it also blocked the effects of exogenous BDNF. BDNF did not enhance the effects of FGF-2 by interacting with it. These experiments defined a temporal sequence in which FGF-2 acts early in development, while BDNF affects a later stage.