DEVELOPMENTAL DISTRIBUTION OF FEMALE-SPECIFIC SEX-LETHAL PROTEINS IN DROSOPHILA-MELANOGASTER
DEVELOPMENTAL DISTRIBUTION OF FEMALE-SPECIFIC SEX-LETHAL PROTEINS IN DROSOPHILA-MELANOGASTER
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DOI：
10.1101/gad.5.3.403
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发表时间：
1991-03-01
影响因子：
10.5
通讯作者：
SCHEDL, P
中科院分区：
文献类型：
--
作者：
BOPP, D;BELL, LR;SCHEDL, P
性别致死（Sxl）的二元开关基因必须在女性和男性关闭，以允许适当的阐述适当的性发育途径在果蝇。先前的研究提出了一种机制，其中Sxl的开/关调节在RNA剪接水平上在转录后发生。该模型的一个关键预测是，功能性Sxl蛋白在男性中不存在，但在女性中存在。在这份报告中，我们表明预期的全长蛋白质只存在于雌性动物中。在女性中发现多种形式的Sxl蛋白，其中一些以阶段和组织特异性模式表达。与Sxl蛋白在调节选择性剪接中的作用一致，所述蛋白定位于细胞核中，在那里它们表现出点状染色模式。令人惊讶的是，几种次要的Sxl蛋白似乎存在于成年男女的特定组织中。这些物种的可能起源进行了讨论。我们还表明，SXL在早期胚胎中的表达是性别特异性的，并取决于母亲的女儿和合子sisterless-B活动，按照这些基因的正调节SXL的既定角色。生殖系中Sxl表达的开始比索马中的晚。
The binary switch gene Sex-lethal (Sxl) must be on in females and off in males to allow the proper elaboration of the appropriate sexual developmental pathway in Drosophila melanogaster. Previous studies suggested a mechanism in which the on/off regulation of Sxl occurs post-transcriptionally at the level of RNA splicing. A critical prediction of this model is that functional Sxl proteins are absent in males but present in females. In this report we show that the expected full-length proteins are only present in female animals. Multiple forms of Sxl protein are found in females, some of which are expressed in a stage- and tissue-specific pattern. Consistent with a role of Sxl proteins in regulating alternate splicing, the proteins are localized in the nucleus where they exhibit a punctate staining pattern. Surprisingly, several minor Sxl proteins appear to be present in specific tissues of both sexes of adults. The possible origin of these species is discussed. We also show that Sxl expression in the early embryo is sex specific and depends on maternal daughterless and zygotic sisterless-b activity in accordance with the established roles of these genes as positive regulators of Sxl. The onset of Sxl expression in the germ line occurs later than that in the soma.