INCREASED RESOLUTION OF LIPOPOLYSACCHARIDES AND LIPOOLIGOSACCHARIDES UTILIZING TRICINE-SODIUM DODECYL SULFATE-POLYACRYLAMIDE GEL-ELECTROPHORESIS
INCREASED RESOLUTION OF LIPOPOLYSACCHARIDES AND LIPOOLIGOSACCHARIDES UTILIZING TRICINE-SODIUM DODECYL SULFATE-POLYACRYLAMIDE GEL-ELECTROPHORESIS
复制标题
DOI：
10.1016/0022-1759(90)90018-q
复制
发表时间：
1990-01-24
影响因子：
2.2
通讯作者：
APICELLA, MA
中科院分区：
文献类型：
--
作者：
LESSE, AJ;CAMPAGNARI, AA;APICELLA, MA
利用新近建立的Tricine-十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（TSDS-PAGE）系统研究了革兰氏阴性菌的脂寡糖（LOS）和脂多糖（LPS）。TSDS-PAGE导致LOS和LPS在“迷你凝胶”形式中的高度分辨率。TSDS-PAGE导致LOS和LPS迁移作为其在电泳过程中的Mr的函数，并允许从蛋白质标准估计Mr。分析了几种LOP。 新描述的程序允许更快速和准确的分析LOS和LPS的核心区域。
We utilized the recently described tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis (TSDS-PAGE) system to study the lipooligosaccharides (LOS) and lipopolysaccharides (LPS) of gram negative bacteria. TSDS-PAGE resulted in a high degree of resolution of LOS and LPS in the ''mini-gel'' format. TSDS-PAGE resulted in the LOS and LPS migrating as a function of their Mr during electrophoresis and allowed estimation of Mr from a protein standard. Several species of LOP were analyzed. The newly described procedure allowed a more rapid and accurate analysis of LOS and the core region of LPS.