Identification of a New Powdery Mildew Resistance Gene pmDHT at or Closely Linked to the Pm5 Locus in the Chinese Wheat Landrace Dahongtou
Identification of a New Powdery Mildew Resistance Gene pmDHT at or Closely Linked to the Pm5 Locus in the Chinese Wheat Landrace Dahongtou
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中国小麦地方品种大红头Pm5基因座上或与之密切相关的新白粉病抗性基因pmDHT的鉴定
DOI：
10.1094/pdis-02-19-0401-re
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发表时间：
2019-10-01
期刊：
影响因子：
4.5
通讯作者：
An, Diaoguo
中科院分区：
文献类型：
--
作者：
Qie, Yanmin;Sheng, Yuan;An, Diaoguo
中国小麦地方品种大红头对 38 个测试的中国小麦白粉病分离株中的 35 个具有抗性。 sp。小麦在幼苗阶段。对大红头与感病品种明县169、徽县红杂交的F-2群体及其衍生的F-2.3家系进行遗传分析表明，大红头对禾谷小孢子虫具有抗性。 sp。小麦分离株E09由单个隐性基因赋予，暂时命名为pmDHT。该基因被定位到染色体臂 7BL，两侧是标记 Xwnw526/XBE443877 和 Xgwm611/Xwmc511，遗传距离分别为 0.8 和 0.3 cM。 pmDHT 的染色体位置与 7BL 上的多等位基因 Pm5 基因座相似。大红头与附庄30号(Pm5e)和小白冬(mlxbd)杂交的等位性测试表明，pmDHT与Pm5e和mlxbd都是等位基因。然而，pmDHT 对 38 B. graminis f 表现出不同的抗性模式。 sp。小麦分离株与带有 Pm5a、Pm5b、Pm5e、mlxbd 和 PmHYM 的小麦品系相比，也与 PmSGA 不同。因此，pmDHT 最有可能被鉴定为新的等位基因或至少是 Pm5 基因座的紧密连锁基因。该基因可以转移到易感小麦品种/品系中，并通过标记辅助选择与其他抗性基因组合，以提高白粉病抗性。
Chinese wheat landrace Dahongtou was resistant to 35 of 38 tested Chinese isolates of Blumeria graminis f. sp. tritici at the seedling stage. Genetic analysis of the F-2 populations and their derived F-2.3 families of crosses of Dahongtou with the susceptible varieties Mingxian 169 and Huixianhong indicated that the resistance of Dahongtou to B. graminis f. sp. tritici isolate E09 was conferred by a single recessive gene, tentatively designated as pmDHT. The gene was mapped to chromosome arm 7BL and flanked by markers Xwnw526/XBE443877 and Xgwm611/Xwmc511 at genetic distances of 0.8 and 0.3 cM, respectively. The chromosomal position of pmDHT was similar to the multi-allelic Pm5 locus on 7BL. Allelism tests with crosses of Dahongtou with Fuzhuang 30 (Pm5e) and Xiaobaidong (mlxbd) indicated that pmDHT was allelic to both Pm5e and mlxbd. However, pmDHT showed a different pattern of resistance to the 38 B. graminis f. sp. tritici isolates compared with wheat lines with Pm5a, Pm5b, Pm5e, mlxbd, and PmHYM and also differed from PmSGA. Thus, pmDHT was identified most likely as a new allele or at least a closely linked gene of the Pm5 locus. This gene can be transferred into susceptible wheat cultivars/lines and pyramided with other resistance genes through marker-assisted selection to improve powdery mildew resistance.