Age-related alterations of gastric mucosa and estrogen synthesis in rat parietal cells
Age-related alterations of gastric mucosa and estrogen synthesis in rat parietal cells
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DOI：
10.1007/s00418-021-02054-0
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发表时间：
2021-11
影响因子：
2.3
通讯作者：
Hiroto Kobayashi;N. Shirasawa;A. Naito
中科院分区：
文献类型：
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作者：
Hiroto Kobayashi;N. Shirasawa;A. Naito
胃除了分泌胃酸外，还有多种功能。多方面的研究调查了胃肠道与年龄相关的变化。然而，对于 3 个月以上大鼠胃壁细胞雌激素产生的变化知之甚少。我们研究了 3 至 24 个月期间胃雌激素合成与年龄相关的变化以及肝脏雌激素受体的相应变化。身体、胃和肝脏的重量从 3 个月到 18 个月线性增加，然后在 24 个月内保持恒定比例。在大鼠的一生中，胃粘膜面积（以 mm2/1 mm 粘膜肌层为单位）显示出恒定的比例。随着年龄的增长，壁细胞H+/K+-ATP酶免疫染色面积逐渐减少。在 3-24 个月时观察到芳香酶抗体免疫阳性的细胞。 18 个月和 24 个月时芳香酶 mRNA 及其蛋白的表达略低于 3 个月时。 12个月时门静脉雌二醇浓度是3个月时的1.5倍，18个月时是3个月时的一半。 18个月和24个月时肝脏中雌激素受体mRNA的表达量约为3个月时的80%。结果表明，随着年龄的增长，胃内雌激素的产生量下降，肝脏雌激素受体也相应受到影响。同时，胃粘膜持续表达芳香酶以维持动物一生的肝功能。
The stomach has diverse functions other than gastric acid secretion. Multifaceted studies have investigated age-related changes of the gastrointestinal tract. Nevertheless, little is known about estrogen production changes in gastric parietal cells in rats aged over 3 months. We investigated age-related changes in gastric estrogen synthesis and the accompanying changes in liver estrogen receptor from 3 to 24 months. Weights of the body, stomach, and liver increased linearly from 3 to 18 months, then maintained a constant proportion up to 24 months. The gastric mucosa area (in mm2/1 mm muscularis mucosa) showed a constant proportion throughout the rats’ life. The population of parietal cells immunostained area with H+/K+-ATPase decreased gradually with advancing age. Cells that were immunopositive to aromatase antibody were observed at 3–24 months. The expressions of aromatase mRNA and its protein were somewhat lower at 18 and 24 months than at 3 months. The portal venous estradiol concentration at 12 months was 1.5 times higher than that at 3 months, and that at 18 months was a half of that at 3 months. The expression of estrogen receptor mRNA in the liver at 18 and 24 months was about 80% of that at 3 months. Results suggest that the gastric estrogen production declines with aging, and the liver estrogen receptor is also affected accordingly. Simultaneously, the gastric mucosa continues to express aromatase to maintain liver function(s) throughout the animal’s life.