Fontibacillus aquaticus gen. nov., sp. nov., isolated from a warm spring.
Fontibacillus aquaticus gen. nov., sp. nov., isolated from a warm spring.
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DOI：
10.1099/ijs.0.012633-0
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发表时间：
2010-02
影响因子：
2.8
通讯作者：
Pradipta Saha;S. Krishnamurthi;A. Bhattacharya;R. Sharma;T. Chakrabarti
中科院分区：
文献类型：
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作者：
Pradipta Saha;S. Krishnamurthi;A. Bhattacharya;R. Sharma;T. Chakrabarti
从温泉水中分离出一种新的兼性厌氧菌株GPTSA 19(T)，并采用多相方法对其进行了表征。革兰氏染色为革兰氏阴性，而氨基肽酶试验为革兰氏阳性。新菌株为嗜中温菌，内孢子呈椭球状。通过16S rRNA基因序列分析，该菌株与motobuensis Paenibacillus MC10(T)的相似性最高（96.0%）。该菌株与其他芽孢杆菌属的基因序列相似性< 95.8%。该菌株还具有PAEN 515F和682F的16S rRNA基因特征序列，这些特征序列通常存在于大多数芽孢杆菌属物种中。该菌株的主要脂肪酸为前异丙酸（15:0），甲基萘醌以MK-7为主。极性脂质包括二磷脂酰甘油（DPG）、磷脂酰甘油（PG）、磷脂酰乙醇胺（PE）、六种未知磷脂（PLs）、一种氨基磷脂（PN）、三种糖脂（GLs）、两种氨基脂（ALs）、一种氨基磷酸糖脂（APGL）和三种未知脂质（ULs）。该新菌株的极性脂质谱，特别是ALs、GLs和PLs，将其与Paenibacillus polymyxa属的公认模式种以及其最近的近亲P. motobuensis区分出来。基于表型和化学分类特征以及16S rRNA基因序列分析，该菌株属于新属，并被命名为Fontibacillus gen. 11 .。新属的模式种为Fontibacillus aquaticus gen. nov., sp. nov.，类型菌株GPTSA 19(T) (=MTCC 7155（T)=DSM 17643(T)）。
A novel facultatively anaerobic strain, designated GPTSA 19(T), was isolated from a warm spring and characterized using a polyphasic approach. The strain behaved as Gram-negative in the Gram staining procedure but showed a Gram-positive reaction in the aminopeptidase test. The novel strain was a mesophilic rod with ellipsoidal endospores. On the basis of 16S rRNA gene sequence analysis, the strain showed closest similarity (96.0 %) with Paenibacillus motobuensis MC10(T). The gene sequence similarity of the novel strain with other species of the genus Paenibacillus was <95.8 %. The novel strain also had PAEN 515F and 682F signature sequence stretches in the 16S rRNA gene that are usually found in most species of the genus Paenibacillus. The strain possessed anteiso-C(15 : 0) as the major fatty acid and MK-7 as the predominant menaquinone. Polar lipids included diphosphatidylglycerol (DPG), phosphatidylglycerol (PG), phosphatidylethanolamine (PE), six unknown phospholipids (PLs), one aminophospholipid (PN), three glycolipids (GLs), two aminolipids (ALs), one aminophosphoglycolipid (APGL) and three unknown lipids (ULs). The polar lipid profile of the novel strain, especially as regards ALs, GLs and PLs, distinguished it from the recognized type species of the genus Paenibacillus, Paenibacillus polymyxa, as well as from its closest relative P. motobuensis. Based on phenotypic and chemotaxonomic characteristics and analysis of the 16S rRNA gene sequence, the new strain merits the rank of a novel genus for which the name Fontibacillus gen. nov. is proposed. The type species of the new genus is Fontibacillus aquaticus gen. nov., sp. nov. with the type strain GPTSA 19(T) (=MTCC 7155(T)=DSM 17643(T)).