Immunohistochemical expression of fibroblast growth factor (FGF)‐2 in epilepsy‐associated malformations of cortical development (MCDs)
Immunohistochemical expression of fibroblast growth factor (FGF)‐2 in epilepsy‐associated malformations of cortical development (MCDs)
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DOI：
10.1111/j.1440-1789.2007.00881.x
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发表时间：
2008-08
期刊：
影响因子：
2.3
通讯作者：
C. Sugiura;H. Miyata;Manami Ueda;E. Ohama;H. Vinters;K. Ohno
中科院分区：
文献类型：
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作者：
C. Sugiura;H. Miyata;Manami Ueda;E. Ohama;H. Vinters;K. Ohno
为探讨异型细胞在皮质发育异常中的生物学意义，用免疫组织化学方法检测了癫痫患者皮质切除标本中成纤维细胞生长因子-2的表达，包括局灶性皮质发育不良(FCD)伴气球细胞(BCS)(n=4；年龄/性别=2m、14m、24m、45m)、结节性硬化症结节(TSC-tubers)(n=2；1F、3F)、FCD(n=3；23F、23m、25m)和胶质样病变(n=3；12m、25m、29m)。所有受检者星形胶质细胞胞核和/或胞浆均为阳性，少突胶质细胞未见表达。在所有异常增生性病变中，均可在星形细胞核、胞浆和/或胞核中检测到FGF2。在有BCS和TSC结节的FCD中，发育不良神经元胞浆内均可见不同程度的阳性表达，而在无BCS的FCD中未见阳性表达。成纤维细胞生长因子-2免疫反应阳性细胞(FGF2-IR%)在伴有BCS的FCD组(46.00±4.1%)明显高于无BCS组(19.8±3.1%)和神经胶质病变组(19.5±3.3%)，差异有统计学意义(P&0.001)。这些结果与以往报道的人胎脑神经母细胞和胶质母细胞，尤其是成人脑内星形胶质细胞的表达相结合，提示成纤维细胞中成纤维细胞生长因子-2的表达反映了发育不良细胞的不完全分化和成熟，而且成纤维细胞生长因子-2-IR%与MCD的组织学亚型有关，反映了每种疾病发生的时间。
To elucidate the biological significance of dysplastic cells in malformations of cortical development, an immunohistochemical study was performed to investigate fibroblast growth factor‐2 (FGF‐2) expression in corticectomy specimens from epilepsy patients, including focal cortical dysplasia (FCD) with balloon cells (BCs) (n = 4; age/sex = 2M, 14F, 24M, 45M), tubers of tuberous sclerosis complex (TSC‐tubers) (n = 2; 1F, 3F), FCD without BCs (n = 3; 23F, 23M, 25M), and gliotic lesions (n = 3; 12M, 25M, 29M). The nucleus and/or cytoplasm of astrocytes in all cases examined were positive for FGF‐2; however, FGF‐2 immunoreactivity was not detected in oligodendroglial cells. In all dysplastic lesions, FGF‐2 was detected in the astrocytic nuclei, and cytoplasm and/or nuclei of BCs. Dysplastic neurons (DNs) in FCD with BCs and TSC‐tubers were variably positive for FGF‐2 in the cytoplasm, but FGF‐2 was not detected in the neurons of FCD without BCs. The number of FGF‐2 immunoreactive cells (FGF‐2‐IR%) in FCD with BCs (46.0 ± 4.1%) was higher than that in FCD without BCs (19.8 ± 3.1%) and gliotic lesions (19.5 ± 3.3%) with statistical significance (P < 0.001). These results, together with previous reports showing FGF‐2 expression in neuroblasts and glioblasts in human fetal brain, and mainly in astrocytes in adult brain, suggest that FGF‐2 expression in MCDs reflects incomplete differentiation and maturation of dysplastic cells, and that FGF‐2‐IR% is associated with histological subtypes of MCD, reflecting the timing of insults underlying the pathogenesis of each disorder.