Indoleamine-2,3-dioxygenase enzyme expression and activity in polarized dendritic cells.
Indoleamine-2,3-dioxygenase enzyme expression and activity in polarized dendritic cells.
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DOI：
10.3109/14653240903271230
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发表时间：
2009
期刊：
影响因子：
4.5
通讯作者：
Ernstoff MS
中科院分区：
文献类型：
--
作者：
Wolf B;Posnick D;Fisher JL;Lewis LD;Ernstoff MS
Polarized mature dendritic cells (DC) can activate cytolytic T-lymphocyte (CTL) responses and may be a more effective clinical strategy in DC-based cancer vaccines. A subset of mature DC can down-regulate the T-cell immune response through expression of indoleamine-2,3-dioxygenase (IDO). We determined whether polarizing DC ex vivo increased IDO expression and activity. Peripheral blood monocytes from healthy volunteers were cultured ex vivo in polarizing and non-polarizing culture conditions. DC IDO expression was detected by Western blot. IDO enzyme activity was determined by high-performance liquid chromatography (HPLC) measurement of kynurenine (K) and tryptophan (T) concentrations in culture supernatants. IDO protein was markedly increased in DC after polarization (median 1222.4%, range 331.5–2113.3%) versus non-polarized DC (median 28.3%, range 3.7–119.8%; P = 0.04). The median K/T ratio was significantly higher in polarized DC versus non-polarized DC (6.34, range 6.02–6.65, versus 0.047, range 0.004–0.541; P = 0.04). IDO protein expression correlated with enzyme activity (r = 0.80, P = 0.002). DC polarizing culture conditions increased expression of IDO protein and IDO enzyme activity. DC culture and maturation methodologies may impact the effectiveness of adoptive DC therapy.
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影响因子：
4.4
作者：
Hwu, P;Du, MX;Young, HA
通讯作者：
Young, HA
影响因子：
3.9
作者：
Wesa, Amy;Kalinski, Pawel;Storkus, Walter J.
通讯作者：
Storkus, Walter J.
影响因子：
5.8
作者：
Wobser, Marion;Voigt, Heike;Becker, Juergen C.
通讯作者：
Becker, Juergen C.
影响因子：
6.4
作者：
Lee, GK;Park, HJ;Mellor, AL
通讯作者：
Mellor, AL
DOI：
10.1084/jem.20020052
发表时间：
2002-08-19
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Terness P;Bauer TM;Röse L;Dufter C;Watzlik A;Simon H;Opelz G
通讯作者：
Opelz G