The genome sequence of Mycoplasma mycoides subsp mycoides SC type strain PG1T, the causative agent of contagious bovine pleuropneumonia (CBPP)
The genome sequence of Mycoplasma mycoides subsp mycoides SC type strain PG1T, the causative agent of contagious bovine pleuropneumonia (CBPP)
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DOI：
10.1101/gr.1673304
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发表时间：
2004-02-01
期刊：
影响因子：
7
通讯作者：
Uhlén, M
中科院分区：
文献类型：
--
作者：
Westberg, J;Persson, A;Uhlén, M
支原体mycoidesSC （MmymySC）是牛传染性胸膜肺炎（CBPP）的病原，CBPP是牛的一种高度传染性呼吸道疾病。对mymy SC型菌株PUT的基因组进行了测序，绘制了所有基因图谱，为进一步研究该生物的细胞功能和CBPP提供了便利。该基因组的特征是一条1,211,703 bp的环状染色体，其G+C含量最低（24摩尔%），插入序列密度最高（占基因组大小的13%）。基因组包含985个假定基因，其中72个是插入序列的一部分并编码转座酶。异常的GC-skew模式和大的重复序列的存在表明高度的基因组可塑性。发现了多种潜在的毒力因素，包括编码推定的可变表面蛋白和酶的基因，以及负责产生过氧化氢和胶囊的转运蛋白，过氧化氢和胶囊被认为对动物有毒性作用。
Mycoplasma mycoides subsp. mycoidesSC (MmymySC) is the etiological agent of contagious bovine pleuropneumonia (CBPP), a highly contagious respiratory disease in cattle. The genome of Mmymy SC type strain PUT has been sequenced to map all the genes and to facilitate further studies regarding the cell function of the organism and CBPP. The genome is characterized by a single circular chromosome of 1,211,703 bp with the lowest G+C content (24 mole%) and the highest density of insertion sequences (13% of the genome size) of all sequenced bacterial genomes. The genome contains 985 putative genes, of which 72 are part of insertion sequences and encode transposases. Anomalies in the GC-skew pattern and the presence of large repetitive sequences indicate a high genomic plasticity. A variety of potential virulence factors was identified, including genes encoding putative variable surface proteins and enzymes and transport proteins responsible for the production of hydrogen peroxide and the capsule, which is believed to have toxic effects on the animal.