Redox status regulates subcelluar localization of PpTGA1 associated with a BABA-induced priming defence against Rhizopus rot in peach fruit
Redox status regulates subcelluar localization of PpTGA1 associated with a BABA-induced priming defence against Rhizopus rot in peach fruit
复制标题
氧化还原状态调节 PpTGA1 的亚细胞定位，与 BABA 诱导的桃果实根霉腐烂防御相关
DOI：
10.1007/s11033-020-05719-6
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发表时间：
2020-08-13
影响因子：
2.8
通讯作者：
Zheng, Yonghua
中科院分区：
文献类型：
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作者：
Li, Chunhong;Wang, Kaituo;Zheng, Yonghua
本研究试图描述氧化还原状态和亚细胞定位的变化在 BABA 诱导的桃果实对根霉腐烂的引发抗性中的作用。具体而言，50 mM BABA 使桃子增强了对匍匐根瘤菌的抗病性，这通过病原体攻击时抑制疾病发展以及 TGA 转录因子 (PpTGA1) 和 NPR1 基因 (PpNPR1) 水平明显升高来证明。此外，BABA诱导增强了PPP和AsA-GSH循环中一系列关键酶的活性，并最终促进NADPH和GSH池的形成，从而将细胞内的氧化还原状态改变为高度还原状态。此外，在没有 BABA 处理或接种 R. stolonifer 的情况下，PpTGA1-GFP 定位于细胞质中，而 BABA 诱导加上 R. stolonifer 接种导致 PpTGA1-GFP 特异性易位到细胞核，在细胞核中与 PpNPR1 相互作用并调节 PR 基因的阳性表达。因此，观察结果表明，BABA 可以促进氧化还原态的还原，导致 PpTGA1 易位到细胞核，这是诱导桃子根霉腐烂防御的先决条件。
This study attempted to characterize the involvement of a change in the redox status and subcellular localization in the BABA-induced priming resistance of peach fruit against Rhizopus rot. Specifically, 50 mM BABA primed the peaches for the enhanced disease resistance against R. stolonifer, as demonstrated by suppression of the disease development upon pathogen challenge accompanied by the clearly elevated level of TGA transcription factor (PpTGA1) and NPR1 gene (PpNPR1). In addition, the BABA elicitation enhanced the activities of a series of critical enzymes in the PPP and AsA-GSH cycle, and eventually promoted the NADPH and GSH pools, which altered the intracellular redox state towards a highly reductive condition. Additionally, PpTGA1-GFP was localized in the cytoplasm in the absence of BABA treatment or R. stolonifer inoculation, while BABA elicitation plus R. stolonifer inoculation caused PpTGA1-GFP to specifically translocate to the nucleus, where it interacted with PpNPR1 and regulated the positive expression of PR genes. Therefore, the observations implied that BABA could promote the reduction of the redox state, resulting in the translocation of PpTGA1 to the nucleus, which was a prerequisite for the induction of a priming defence against Rhizopus rot in peach.