The epigenetic landscapes of histone modifications on HSV-1 genome in human THP-1 cells
The epigenetic landscapes of histone modifications on HSV-1 genome in human THP-1 cells
复制标题
人类 THP-1 细胞中 HSV-1 基因组组蛋白修饰的表观遗传景观。
DOI：
10.1016/j.antiviral.2020.104730
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发表时间：
2020-04-01
影响因子：
7.6
通讯作者：
Li, Lian-Yun
中科院分区：
文献类型：
--
作者：
Gao, Chuan;Chen, Lin;Li, Lian-Yun
组蛋白在病毒基因组上的定位和修饰是调节病毒复制的重要因素。为了研究组蛋白修饰在病毒基因组上的动态及其在抗病毒反应中的潜在作用，我们在THP-1细胞中研究了HSV-1基因组中组蛋白修饰的动态变化。HSV-1基因组在感染后不久检测到组蛋白修饰，包括H3K9me3、H3K27me3、H3K4me3和H3K27ac。这些修饰在感染后不久就出现在病毒基因组上，并随着病毒生命周期的进展而迅速变化。在感染过程中，病毒基因组上的转录抑制标记H3K9me3和H3K27me3减少，转录激活标记H3K27ac增加。H3K27ac转移酶p300的抑制剂C646可显著抑制病毒复制和病毒基因表达。我们的研究揭示了组蛋白修饰与病毒基因表达之间的关系，并为抗病毒治疗提供了潜在的新策略。
Histone positioning and modifications on viral genomes are important factors regulating virus replication. To investigate the dynamics of modified histones on the viral genome and their potential roles in antiviral response, we studied the dynamic changes of histone modifications across the HSV-1 genome in THP-1 cells. Histone modifications were detected on the HSV-1 genome soon after infection, including H3K9me3, H3K27me3, H3K4me3 and H3K27ac. These modifications emerged on the viral genome soon after infection and changed rapidly along with virus life cycle progression. The transcription repression marks, H3K9me3 and H3K27me3, decreased on the viral genome during the infection process; the transcription activation mark H3K27ac increased. Treatment with C646, an inhibitor of H3K27ac transferase p300, significantly repressed virus replication and viral gene expression. Our study reveals the relationship between histone modifications and viral gene expression and provides potential novel strategies for antiviral treatment.