Fluoride reduces the expression of enamel proteins and cytokines in an ameloblast-derived cell line
Fluoride reduces the expression of enamel proteins and cytokines in an ameloblast-derived cell line
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DOI：
10.1016/j.archoralbio.2010.10.024
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发表时间：
2011-04-01
影响因子：
3
通讯作者：
Reseland, Janne E.
中科院分区：
文献类型：
--
作者：
Riksen, Elisabeth Aurstad;Kalvik, Anne;Reseland, Janne E.
目的：探讨两种不同浓度的氟对成釉细胞来源的细胞株表达釉蛋白、碱性磷酸酶（ALP）、细胞因子和白细胞介素的影响。方法：将小鼠成釉细胞来源的细胞（LS-8），小鼠牙源性上皮细胞，暴露于1或5 ppm氟化钠（NaF）（0.46和2.25 ppm F，分别）1，3和7天。量化氟化钠对釉质蛋白mRNA表达的影响;测量细胞因子和白细胞介素的分泌以及碱性磷酸酶（ALP）活性，并与未处理的对照进行比较。使用BrdU incorporation.Results：氟化物在2.25 ppm的结构釉基质蛋白釉原蛋白（amelogenin），成釉蛋白（ameloblastin），釉蛋白（enamelin），和釉蛋白酶基质金属肽酶-20（MMP-20）的mRNA表达减少后，1-和3-天的细胞生长的影响进行了测量。类似地，几种血管形成因子（血管内皮生长因子（VEGF）、单核细胞趋化蛋白（MCP-1）和干扰素诱导蛋白10（IP-10））也被2.25 ppm氟化物降低。ALP活性和增殖的刺激0.46 ppm的氟化物，但抑制2.25 ppm的fluorine.Conclusions：这些结果表明，氟化物可能会影响釉质结构蛋白的表达和蛋白酶负责处理这些蛋白质在分泌阶段的成釉和去某种程度上解释矿化缺陷，特点氟斑牙釉质。(C)2010爱思唯尔有限公司保留所有权利。
Objective: To investigate the effects of two different fluoride concentrations on the expression of enamel proteins, alkaline phosphatase (ALP), cytokines and interleukins by an ameloblast-derived cell line.Methods: Murine ameloblast-derived cells (LS-8), mouse odontogenic epithelia, were exposed to 1 or 5 ppm sodium fluoride (NaF) (0.46 and 2.25 ppm F, respectively) for 1, 3 and 7 days. The effect of NaF on the mRNA expression of enamel proteins was quantified; the secretion of cytokines, and interleukins, and the alkaline phosphatase (ALP) activity, into the cell culture medium was measured and compared to untreated controls. The effect on cell growth after 1- and 3-days in culture was measured using BrdU incorporation.Results: Fluoride at 2.25 ppm reduced mRNA expression of the structural enamel matrix proteins amelogenin (amel), ameloblastin (ambn), enamelin (enam), and the enamel protease matrix metallopeptidase-20 (MMP-20). Similarly several vascularisation factors (vascular endothelial growth factor (VEGF), monocyte chemoattractant proteins (MCP-1) and interferon inducible protein 10 (IP-10), was also reduced by 2.25 ppm fluoride. ALP activity and proliferation were stimulated by 0.46 ppm fluoride but inhibited by 2.25 ppm fluoride.Conclusions: These results indicate that fluoride may impact on the expression of structural enamel proteins and the protease responsible for processing these proteins during the secretory stage of amelogenesis and go some way to explaining the mineralization defect that characterises fluorotic enamel. (C) 2010 Elsevier Ltd. All rights reserved.