Slug is a mediator of epithelial-mesenchymal cell transformation in the developing chicken heart
Slug is a mediator of epithelial-mesenchymal cell transformation in the developing chicken heart
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DOI：
10.1006/dbio.1999.9339
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发表时间：
1999-08-01
影响因子：
2.7
通讯作者：
Runyan, RB
中科院分区：
文献类型：
--
作者：
Romano, LA;Runyan, RB
心脏房室 (AV) 管中心内膜垫的发育过程中会发生上皮间充质细胞转化。我们假设这种上皮-间质细胞转化需要转录因子Slug，因为鸡胚原肠胚形成和神经嵴分化期间的类似转化需要Slug。我们通过RT-PCR和免疫染色发现Slug在鸡胚心脏中的时间和空间定位与Slug在心内膜垫形成中的作用一致。此外，我们发现 AV 管内皮细胞表达的 Slug 是由 AV 管心肌提供的信号诱导的。 Slug 似乎是鸡心脏上皮间质细胞转化所必需的，因为用反义 Slug 寡脱氧核苷酸处理 AV 管外植体可抑制体外间充质细胞的形成。反义Slug寡脱氧核苷酸阻止内皮细胞-细胞分离，表明Slug在转化途径的早期发挥作用。 (C) 1999 年学术出版社。
An epithelial-mesenchymal cell transformation occurs during the development of the endocardial cushions in the atrioventricular (AV) canal of the heart. We hypothesized that the transcription factor Slug is required for this epithelial-mesenchymal cell transformation since Slug is required for similar transformations during gastrulation and neural crest differentiation in chicken embryos. We found by RT-PCR and immunostaining that the temporal and spatial localization of Slug in the embryonic chicken heart is consistent with a role for Slug in endocardial cushion formation. Moreover, we found that Slug expression by AV canal endothelial cells is induced by a signal provided by AV canal myocardium. Slug appears to be required for epithelial-mesenchymal cell transformation in the chicken heart since treatment of AV canal explants with antisense Slug oligodeoxynucleotides inhibited mesenchymal cell formation in vitro. Antisense Slug oligodeoxynueleotides prevented endothelial cell-cell separation, suggesting that Slug acts early in the transformation pathway. (C) 1999 Academic Press.