DEVELOPMENT OF MULTIPURPOSE PEROXISOMES IN CANDIDA-BOIDINII GROWN IN OLEIC ACID-METHANOL LIMITED CONTINUOUS CULTURES
DEVELOPMENT OF MULTIPURPOSE PEROXISOMES IN CANDIDA-BOIDINII GROWN IN OLEIC ACID-METHANOL LIMITED CONTINUOUS CULTURES
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DOI：
10.1128/jb.174.12.4057-4063.1992
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发表时间：
1992-06-01
影响因子：
3.2
通讯作者：
VEENHUIS, M
中科院分区：
文献类型：
--
作者：
WATERHAM, HR;KEIZERGUNNINK, I;VEENHUIS, M
我们研究了过氧化物酶体在酵母假丝酵母（Candida boidinii）适应培养条件后的发育和代谢意义，这些条件需要两个独立的过氧化物酶体介导的生长途径同时存在和活性。在油酸生长的细胞中加入甲醇后，在指数生长后期，产生了许多新的小过氧化物酶体，除了存在β -氧化酶外，其特征是存在参与甲醇代谢的酶（醇氧化酶和二羟丙酮合成酶）。然而，后一种蛋白质在较大的细胞器中不存在，这些细胞器在加入甲醇之前最初存在于油酸培养的细胞中，并且只包含β -氧化途径的酶。在油酸和甲醇混合物中连续培养的细胞在稳态条件下进行的后续实验表明，β -氧化途径的酶和参与甲醇代谢的酶都在同一个隔间中被发现。因此，在这些条件下，细胞含有过氧化物酶体，它们同时参与两种不同碳源的代谢，同时用于生长。我们的研究结果表明，在添加甲醇后的瞬时状态下观察到的单细胞过氧化物酶体群体的异质性只是暂时的，并且是由于这些细胞器之间结合新合成的基质蛋白的能力的异质性。
We have studied the development and metabolic significance of peroxisomes in the yeast Candida boidinii following adaptation of the organism to cultivation conditions which require the simultaneous presence and activity of two independent peroxisome-mediated pathways for growth. After the addition of methanol to oleic acid-grown cells at late exponentional growth, a number of new small peroxisomes developed which, apart from the presence of beta-oxidation enzymes, were characterized by the presence of enzymes involved in methanol metabolism (alcohol oxidase and dihydroxyacetone synthase). The latter proteins, however, were absent in the larger organelles which were originally present in the oleic acid-grown cells prior to the addition of methanol and which contained only enzymes of the beta-oxidation pathway. Subsequent experiments on cells from continuous cultures grown on a mixture of oleic acid and methanol at steady-state conditions revealed that both the enzymes of the beta-oxidation pathway and those involved in methanol metabolism were found in one and the same compartment. Thus, under these conditions the cells contained peroxisomes which were concurrently involved in the metabolism of two different carbon sources simultaneously used for growth. Our results indicated that the heterogeneity in the peroxisomal population of a single cell, observed in the transient state following the addition of methanol, is only temporary and due to heterogeneity among these organelles with respect to their capacity to incorporate newly synthesized matrix proteins.