Targeting N-acetylglucosamine-bearing polymer-coated liposomes to vascular smooth muscle cells
Targeting N-acetylglucosamine-bearing polymer-coated liposomes to vascular smooth muscle cells
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将带有 N-乙酰氨基葡萄糖的聚合物包被的脂质体靶向血管平滑肌细胞
DOI：
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发表时间：
2011
影响因子：
1.3
通讯作者：
Uichi Ikeda
中科院分区：
文献类型：
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作者：
Mamiko Ise;H. Ise;Y. Shiba;Satoshi Kobayashi;M. Goto;Masafumi Takahashi;T. Akaike;Uichi Ikeda
靶向给药治疗经皮冠状动脉介入治疗后再狭窄具有很大的治疗潜力。为了开发一种靶向损伤血管的药物递送系统，我们研究了含n -乙酰氨基葡萄糖（GlcNAc）的聚合物包被脂质体（GlcNAc- ls）是否被血管平滑肌细胞（VSMCs）特异性摄取。流式细胞术分析显示，体外培养的VSMCs对GlcNAc-Ls有吸收。此外，将glcnac - l静脉注射给钢丝介导的血管损伤小鼠。glcnac - l在损伤血管壁内明显积聚，而在对侧（未损伤）血管壁不明显积聚。这些结果表明，GlcNAc-Ls在体外和体内均可被VSMCs特异性吸收。我们提出了一种使用GlcNAc-Ls的新策略，该策略有可能应用于靶向损伤血管的药物递送。
The targeted delivery of anti-inflammatory agents has great therapeutic potential for treating restenosis following percutaneous coronary intervention. To develop a drug delivery system targeted to injured blood vessels, we examined whether N-acetylglucosamine (GlcNAc)-bearing polymer-coated liposomes (GlcNAc-Ls) are specifically taken up by vascular smooth muscle cells (VSMCs). Flow cytometric analysis revealed that GlcNAc-Ls were taken up by VSMCs in vitro. Furthermore, GlcNAc-Ls were intravenously administered to mice that had undergone wire-mediated vascular injury. GlcNAc-Ls markedly accumulated at the intramural site of the injured vessel walls but not at the contralateral (uninjured) vessel walls. These results demonstrated that GlcNAc-Ls can be specifically taken up by VSMCs both in vitro and in vivo. We propose a novel strategy of using GlcNAc-Ls that has potential for application in drug delivery targeted to injured blood vessels.