Eimeria tenella:: Construction of a recombinant fowlpox virus expressing rhomboid gene and its protective efficacy against homologous infection
Eimeria tenella:: Construction of a recombinant fowlpox virus expressing rhomboid gene and its protective efficacy against homologous infection
复制标题
柔嫩艾美耳球虫：表达菱形基因的重组鸡痘病毒的构建及其对同源感染的保护作用。
DOI：
10.1016/j.exppara.2007.12.009
复制
发表时间：
2008-05-01
影响因子：
2.1
通讯作者：
Gong, Pengtao
中科院分区：
文献类型：
--
作者：
Yang, Guilian;Li, Jianhua;Gong, Pengtao
构建了表达柔嫩艾美耳球虫菱形体基因的重组鸡痘病毒（rFPV），并测定了其对同源感染鸡的保护效果。将三日龄的无特异性病原体（SPF）鸡皮下免疫。用10（2）空斑形成单位（PFU）、10（4）PFU或10（6）PFU的rFPV-菱形感染，并在免疫后（p.i.）14天用5 × 10（4）同源孢子化卵囊攻击。免疫后1、2、3和4周测定特异性抗体应答和淋巴细胞增殖。通过测定卵囊排出量、体重增长和病变评分来评价免疫保护效果。rFPV-rhomboid可引起特异性体液免疫反应并刺激外周血淋巴细胞增殖。rFPV-rhomboid免疫组的病变评分显著高于其他组。与其他组相比，rFPV-rhomboid组体重明显增加。rFPV-rhomboid免疫后，卵囊脱落率显著降低，以10（2）PFU、10（4）PFU和10（6）PFU剂量免疫的保护率分别为39.6%、41.1%和41.7%。这些结果表明，rFPV能诱导鸡产生免疫应答，对柔嫩艾美耳球虫攻击具有部分保护作用。(C)2008年爱思唯尔公司All rights reserved.
A recombinant fowlpox virus (rFPV) expressing the Eimeria tenella rhomboid gene was constructed and its protective efficacy against homologous infection in chickens determined. Three-day-old-specific pathogen free (SPF) chickens were immunized s.c. with 10(2) plaque forming units (PFU), 10(4) PFU, or 10(6) PFU of rFPV-rhomboid, and challenged with 5 x 10(4) homologous sporulated oocysts 14 days post-immunization (p.i.). The specific antibody response and lymphocyte proliferation were measured 1, 2, 3 and 4 weeks p.i. Oocyst output, body weight gains and lesion scores were measured to evaluate the protective effects of immunization. rFPV-rhomboid elicited a specific humoral immune response and stimulated proliferation of peripheral blood lymphocytes. The lesion scores in groups vaccinated with rFPV-rhomboid were significantly higher than in other groups. At the same time, rFPV-rhomboid improved body weight significantly compared with other groups. Immunization with rFPV-rhomboid reduced oocyst shedding significantly, resulting in a protection rate of 39.6%, 41.1% or 41.7% given a dose of 10(2) PFU, 10(4) PFU, or 10(6) PFU of rFPV-rhomboid, respectively. These results indicated that rFPV can induce immune responses and offer partial protection of chickens against E tenella challenge. (C) 2008 Elsevier Inc. All rights reserved.