Molecular mapping of Yr53, a new gene for stripe rust resistance in durum wheat accession PI 480148 and its transfer to common wheat
Molecular mapping of Yr53, a new gene for stripe rust resistance in durum wheat accession PI 480148 and its transfer to common wheat
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DOI：
10.1007/s00122-012-1998-0
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发表时间：
2012-10
影响因子：
5.4
通讯作者：
Liangsheng Xu;Liangsheng Xu;Mei-nan Wang;Peng Cheng;Z. Kang;S. Hulbert;Xianming Chen;Xianming Chen
中科院分区：
文献类型：
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作者：
Liangsheng Xu;Liangsheng Xu;Mei-nan Wang;Peng Cheng;Z. Kang;S. Hulbert;Xianming Chen;Xianming Chen
条锈病，由条锈菌引起。小麦赤霉病（Pst）是世界范围内危害小麦最严重的病害之一。因此，必须找到新的基因来有效地抵抗这种疾病。硬质小麦PI 480148，最初来自埃塞俄比亚，在受控温室条件下和在多个地点经受数年的自然感染的情况下，在美国用几种主要Pstraces进行的所有幼苗测试中是抗性的。为了定位抗性基因并将其转移到普通小麦中，在PI 480148和感病普通小麦基因型Avocet S（AvS）之间进行杂交。通过细胞学和PstracePST-100鉴定，筛选出42条染色体的F_3代抗性植株。用PST-100对2n= 42的F3代植株进行抗性鉴定，共获得157株F4代植株，抗性与感病的比例为3 ∶ 1，表明来自PI 480148的单一显性基因赋予抗性。利用F3：4群体和抗病基因类似物多态性（RGAP）和简单重复序列（SSR）标记，将该基因定位在2B染色体长臂上。SSR标记Xwmc 441和RGAP标记XLRRrev/NLRRrev 350分别位于抗病基因两侧5.6和2.7cM处。该基因对Yr 5强毒的澳大利亚分离物的有效抗性也位于2BL上，对所有的美国品种都有抗性，同时对两个基因的等位性进行了检测，表明来自PI 480148的基因不同于Yr 5，可能是一个新的有用的抗条锈基因。选择具有与AvS相似的植物类型的抗性普通小麦品系用于育种计划。
Stripe rust, caused byPuccinia striiformisf. sp.tritici(Pst), is one of the most damaging diseases of wheat worldwide. It is essential to identify new genes for effective resistance against the disease. Durum wheat PI 480148, originally from Ethiopia, was resistant in all seedling tests with several predominantPstraces in the US under controlled greenhouse conditions and at multiple locations subject to natural infection for several years. To map the resistance gene(s) and to transfer it to common wheat, a cross was made between PI 480148 and susceptible common wheat genotype Avocet S (AvS). Resistant F3plants with 42 chromosomes were selected cytologically and by testing withPstrace PST-100. A total of 157 F4plants from a single F3plant with 2n= 42 tested with PST-100 segregated in a 3 resistant: 1 susceptible ratio, indicating that a single dominant gene from PI 480148 conferred resistance. Using the F3:4population and the resistance gene-analog polymorphism (RGAP) and simple sequence repeat (SSR) markers, the gene was mapped to the long arm of chromosome 2B. SSR markerXwmc441and RGAP markerXLRRrev/NLRRrev350flanked the resistance gene by 5.6 and 2.7 cM, respectively. The effective resistance of the gene to an AustralianPstisolate virulent toYr5, which is also located on 2BL and confers resistance to all USPstraces, together with an allelism test of the two genes, indicated that the gene from PI 480148 is different fromYr5and should be a new and useful gene for resistance to stripe rust. Resistant common wheat lines with plant types similar to AvS were selected for use in breeding programs.