Comprehensive insights into host-pathogen interaction between brown planthopper and a fungal entomopathogen by dual RNA sequencing.
Comprehensive insights into host-pathogen interaction between brown planthopper and a fungal entomopathogen by dual RNA sequencing.
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DOI：
10.1002/ps.6529
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发表时间：
2021-06
影响因子：
4.1
通讯作者：
Zheng-Liang Wang;Hai-bo Pan;Mu-Yu Li;Wei Wu;Xiaoping Yu
中科院分区：
文献类型：
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作者：
Zheng-Liang Wang;Hai-bo Pan;Mu-Yu Li;Wei Wu;Xiaoping Yu
褐飞虱（brownplanthopper，BPH）是水稻上最具破坏性的害虫之一，每年造成巨大的产量和经济损失。金龟子绿僵菌（Metarhiziumanisopliae）是一种具有很好生物防治潜力的真菌。全基因组范围内的昆虫-真菌相互作用的洞察是至关重要的遗传改良的M。绿僵菌，以提高其对BPH的毒力，但仍然很少探索。结果使用双重RNA-seq方法，我们在这里呈现了在真菌感染期间BPH成人中宿主和真菌基因表达的全局视图。结果表明，BPH可通过上调角质层形成、免疫反应、细胞解毒和生物大分子代谢等基因的表达，启动对真菌攻击的强烈防御反应。相应地，真菌昆虫病原菌可以诱导一系列基因感染并调控BPH，包括真菌侵入、侵入生长、抗逆和毒力等相关基因。选择了3个与宿主防御相关的基因（NlPCE 4、NlPOD 1和NlCYP 4DE 1）进行功能分析。RNAi介导的NlPCE 4的敲低导致BPH存活率显著降低，但通过抑制NlPOD 1和NlCYP 4DE 1未检测到对存活率的明显影响。dsRNA注射和真菌感染的组合可以显著提高对BPH的杀灭速度，因为在RNAi和M.绿僵菌感染结论本研究为深入研究BPH与M之间宿主-病原相互作用的分子机制提供了新的思路。本研究为今后开发新的有效的生物防治策略提供了理论依据。
BACKGROUND The brown planthopper (BPH) is one of the most destructive pests of rice, causing tremendous yield and economic losses every year. The fungal entomopathogen Metarhizium anisopliae was previously proved to have great potential for BPH biocontrol. Genome-wide insight into the insect-fungus interaction is crucial for genetic improvement of M. anisopliae to enhance its virulence to BPH but still has been poorly explored. RESULTS Using dual RNA-seq approach, we present here a global view of host and fungal gene expressions in BPH adults during the fungal infection. The results revealed that BPH could initiate strong defense responses against the fungal attack by upregulating the expressions of a large number of genes, including genes involved in cuticle formation, immune response, cell detoxification and biomacromolecule metabolism. Correspondingly, the fungal entomopathogen could induce a series of genes to infect and modulate BPH, including genes involved in fungal penetration, invasive growth, stress resistance and virulence. Three host defense-related genes (NlPCE4, NlPOD1 and NlCYP4DE1) were chose for further function analysis. RNAi-mediated knockdown of NlPCE4 caused a significant decrease in BPH survival, but no obvious effects on the survival rates were detected by the suppression of NlPOD1 and NlCYP4DE1. Combination of dsRNA injection and fungal infection could significantly enhance the BPH-killing speed, as synergistic mortalities were observed in co-treatments of RNAi and M. anisopliae infection. CONCLUSION Our study provides a comprehensive insight into molecular mechanisms of host-pathogen interaction between BPH and M. anisopliae and contributes to future development of new efficient biocontrol strategies for BPH biocontrol.