Alginate Hydrogel Conglutinate Cells on the Surface of Polycaprolactone Vascular Scaffolds Fabricated by Electrospinning
Alginate Hydrogel Conglutinate Cells on the Surface of Polycaprolactone Vascular Scaffolds Fabricated by Electrospinning
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海藻酸盐水凝胶将细胞粘附在静电纺丝聚己内酯血管支架表面
DOI：
10.1166/jbt.2015.1283
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发表时间：
2015
影响因子：
0.1
通讯作者：
周敏
中科院分区：
文献类型：
--
作者：
周敏
开发一种新型的人工血管支架细胞接种技术。方法将脂肪干细胞分化而来的大鼠内皮祖细胞通过海藻酸盐水凝胶粘连细胞(AHCCs)和自然沉降接种细胞(NSSCs)接种到静电纺丝聚己内酯制作的支架表面。分别培养1、3、7天后，通过免疫荧光和扫描电镜观察细胞形态。结果在同一时间点，AHCC支架比NSSC支架有更多的粘附和增殖。 AHCC组细胞可以直接粘附在血管支架上，避免了NSSC组细胞粘附前的时间延迟。结论海藻酸盐水凝胶粘连细胞有效地接种在血管支架上，可以避免细胞粘附前的时间延迟。
To develop a novel cell-seeding technique on artificial vascular scaffolds.MethodsThe rat endothelial progenitor cells differentiated from adipose-derived stem cells were seeded on the surface of scaffold fabricated by electrospinning polycaprolactone through alginate hydrogel conglutinate cells (AHCCs) and natural sedimentation seeding cells (NSSCs). The cell morphology was observed by immunofluorescence and scanning electron microscopy after cultivation for 1, 3, 7 days, respectively.ResultsMore adhesion and proliferation was noticed in AHCC scaffolds than NSSC scaffolds at the same time point. In the AHCC group, cells could adhere directly on the vascular scaffolds, avoiding the time delay in the NSSC group prior to cell adhesion.ConclusionAlginate hydrogel conglutinate cells are effectively seeded on the vascular scaffolds, which could avoid the time delay before cell adhesion.