Reverse iontophoresis of L-lactate: in vitro and in vivo studies.
Reverse iontophoresis of L-lactate: in vitro and in vivo studies.
复制标题
L-乳酸的反向离子电渗疗法：体外和体内研究。
DOI：
10.1002/jps.20989
复制
发表时间：
2007
影响因子：
3.8
通讯作者：
Guy,RichardH
中科院分区：
文献类型：
--
作者：
Nixon,Susan;Sieg,Anke;Delgado-Charro,MB;Guy,RichardH
This work investigates the reverse iontophoretic extraction of lactate, a widely used marker of tissue distress in critically ill patients and of sports performance.In vitroexperiments were performed to establish the relationship between subdermal lactate levels and lactate iontophoretic extraction fluxes. Subsequently, the iontophoretic extraction of lactate was performedin vivoin healthy volunteers. Lactate was quickly and easily extracted by iontophoresis bothin vitroandin vivo. During a short initial phase, iontophoresis extracts the lactate present in the skin reservoir, providing information of relevance, perhaps, for dermatological and cosmetic applications. In a second step, lactate is extracted from the interstitial subdermal fluid allowing local lactate kinetics to be followed in a completely non‐invasive way. The simultaneousin vivoextraction of chloride, and its possible role as an internal standard to calibrate lactate reverse iontophoretic fluxes, was also demonstrated. Despite these positive findings, however, considerably more research is necessary to eliminate potential artefacts and to facilitate interpretation of the data. © 2007 Wiley‐Liss, Inc. and the American Pharmacists Association J Pharm Sci 96: 3457–3465, 2007
