Generation of cell hybrids via a fusogenic cell tine
Generation of cell hybrids via a fusogenic cell tine
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DOI：
10.1002/jgm.906
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发表时间：
2006-07-01
影响因子：
3.5
通讯作者：
Brandenburger, Annick
中科院分区：
文献类型：
--
作者：
Cheong, Siew Chiat;Blangenois, Isabelle;Brandenburger, Annick
背景 通过肿瘤细胞（TC）和树突状细胞（DC）融合获得的杂交体已被提议作为抗肿瘤疫苗，因为它们具有将肿瘤相关抗原的表达与有效的抗原呈递相结合的潜力。用于融合的经典方法，聚乙二醇 (PEG) 和电融合，具有细胞毒性，并产生可被 DC 吸收的细胞碎片，使得鉴定真正的杂交体变得困难。方法我们建立了表达病毒融合膜糖蛋白 (FMG) 的稳定细胞系，该细胞本身不易融合。该细胞系已用于产生杂交体并评估用于杂交检测的工具的相关性。结果该表达FMG的细胞系促进自体或同种异体TC和DC之间以任意组合的融合，产生“三亲杂交体”。发现至少 20% 的 TC 整合到杂交体中。 结论 据推测，这种三亲杂交方法为进一步调节 DC/TC 杂交体的抗肿瘤作用提供了新的可能性，因为它允许通过适当的融合细胞系工程来表达相关的免疫刺激分子。版权所有 (c) 2006 John Wiley & Sons, Ltd.
Background Hybrids obtained by fusion between tumour cells (TC) and dendritic cells (DC) have been proposed as anti-tumour vaccines because of their potential to combine the expression of tumour-associated antigens with efficient antigen presentation. The classical methods used for fusion, polyethylene glycol (PEG) and electrofusion, are cytotoxic and generate cell debris that can be taken up by DC rendering the identification of true hybrids difficult.Methods We have established a stable cell line expressing a viral fusogenic membrane glycoprotein (FMG) that is not itself susceptible to fusion. This cell line has been used to generate hybrids and to evaluate the relevance of tools used for hybrid detection.Results This FMG-expressing cell line promotes fusion between autologous or allogeneic TC and DC in any combination, generating 'tri-parental hybrids'. At least 20% of TC are found to be integrated into hybrids.Conclusions It is speculated that this tri-parental hybrid approach offers new possibilities to further modulate the anti-tumour effect of the DC/TC hybrids since it allows the expression of relevant immunostimulatory molecules by appropriate engineering of the fusogenic cell line. Copyright (c) 2006 John Wiley & Sons, Ltd.