Role of a Ubiquitously Expressed Receptor in the Vertebrate Olfactory System
Role of a Ubiquitously Expressed Receptor in the Vertebrate Olfactory System
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DOI：
10.1523/jneurosci.2339-13.2013
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发表时间：
2013-09-18
影响因子：
5.3
通讯作者：
Ngai, John
中科院分区：
文献类型：
--
作者：
DeMaria, Shannon;Berke, Allison P.;Ngai, John
气味线索由嗅觉感觉神经元上表达的受体识别，嗅觉感觉神经元是嗅觉上皮的主要感觉神经元。气味受体通常遵循“一个受体，一个神经元”规则，其中嗅觉神经元的感受野由其表达的单一气味受体决定。然后，大脑解释嗅觉神经元群体中气味诱发的受体活动，以识别气味刺激的分子性质。在本研究中，我们表征了 C 家族 G 蛋白偶联受体的特性，与大多数其他气味受体不同，该受体在斑马鱼嗅上皮和小鼠犁鼻器的大量微绒毛感觉神经元中表达。我们发现斑马鱼中的这种受体 OlfCc1 及其鼠类直系同源物 Vmn2r1 是一种钙依赖性、低敏感性受体，对疏水性氨基酸异亮氨酸、亮氨酸和缬氨酸具有特异性。斑马鱼胚胎的功能丧失实验表明，OlfCc1 是对极性、非极性、酸性和碱性氨基酸的多种混合物的嗅觉反应所必需的。还发现 OlfCc1 可以促进其他 OlfC 受体家族成员定位到异源细胞的质膜上。总之，这些结果表明广泛表达的 OlfCc1 是嗅觉系统检测氨基酸所必需的，并表明它在与其共表达的其他嗅觉和犁鼻受体的功能和/或细胞内运输中发挥作用。
Odorant cues are recognized by receptors expressed on olfactory sensory neurons, the primary sensory neurons of the olfactory epithelium. Odorant receptors typically obey the "one receptor, one neuron" rule, in which the receptive field of the olfactory neuron is determined by the singular odorant receptor that it expresses. Odor-evoked receptor activity across the population of olfactory neurons is then interpreted by the brain to identify the molecular nature of the odorant stimulus. In the present study, we characterized the properties of a C family G-protein-coupled receptor that, unlike most other odorant receptors, is expressed in a large population of microvillous sensory neurons in the zebrafish olfactory epithelium and the mouse vomeronasal organ. We found that this receptor, OlfCc1 in zebrafish and its murine ortholog Vmn2r1, is a calcium-dependent, low-sensitivity receptor specific for the hydrophobic amino acids isoleucine, leucine, and valine. Loss-of-function experiments in zebrafish embryos demonstrate that OlfCc1 is required for olfactory responses to a diverse mixture of polar, nonpolar, acidic, and basic amino acids. OlfCc1 was also found to promote localization of other OlfC receptor family members to the plasma membrane in heterologous cells. Together, these results suggest that the broadly expressed OlfCc1 is required for amino acid detection by the olfactory system and suggest that it plays a role in the function and/or intracellular trafficking of other olfactory and vomeronasal receptors with which it is coexpressed.