Development of EST-SSR in foxtail millet (Setaria italica)
Development of EST-SSR in foxtail millet (Setaria italica)
复制标题
DOI：
10.1007/s10722-006-9139-8
复制
发表时间：
2007-03-01
影响因子：
2
通讯作者：
Li, Yu
中科院分区：
文献类型：
--
作者：
Jia, Xiao-Ping;Shi, Yun-Su;Li, Yu
本文报道了在谷子（Setaria italica）中开发EST-SSR的多态性和可转移性研究。从1213条EST序列中获得30条ssr，设计了26条引物。其中，4对SSR引物在12个谷子品种和1个谷子野生近缘种蛇尾草（Setaria viridis）中扩增出多态性产物，4个位点共检测到10个等位基因，每个位点检测到2.5个等位基因。另外，有10个SSR标记可以转移到其他9个禾本科植物上。鉴定了11个含有多态性和可转移SSRs的est的推测功能。
The development of EST-SSR in foxtail millet (Setaria italica) for polymorphism and transferability study was reported here. From 1213 EST sequences, 30 SSRs were obtained and primers were designed for 26 SSRs. Among them, four pairs of SSR primers amplified polymorphic products in 12 foxtail millet cultivars and one accession of Setaria viridis, a wild relative of foxtail millet, with 10 alleles detected for the four loci and 2.5 alleles per locus. In addition, ten SSR markers could be transferred to other nine Gramineae species. The putative functions of 11 ESTs containing polymorphic and transferable SSRs were also identified.