Optical electrocorticogram (OECoG) using wide-field calcium imaging reveals the divergence of neuronal and glial activity during acute rodent seizures
Optical electrocorticogram (OECoG) using wide-field calcium imaging reveals the divergence of neuronal and glial activity during acute rodent seizures
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DOI：
10.1016/j.yebeh.2015.04.036
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发表时间：
2015-08-01
影响因子：
2.6
通讯作者：
Schwartz, Theodore H.
中科院分区：
文献类型：
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作者：
Daniel, Andy G. S.;Laffont, Philippe;Schwartz, Theodore H.
神经胶质在癫痫中的作用一直备受争议。利用体内大量装载的钙染料，我们成像的神经元和神经胶质活性在急性药物啮齿动物模型的新皮质癫痫发作。光学钙基ECoG图显示神经元波传播迅速，大部分仍局限于癫痫发作灶。神经胶质波在癫痫发作时被触发，但以一种典型的方式缓慢地传播，远远超出了癫痫发作的病灶。虽然它们在发病时是相关的，但这两种现象在癫痫发作演变过程中的分歧使人们对它们的相互依赖性和神经胶质在癫痫发作和神经血管耦合中的重要作用产生了疑问。(C) 2015爱思唯尔公司版权所有，
The role of glia in epilepsy has been widely debated. Using in vivo bulk loading of calcium dyes, we imaged neuronal and glial activity in an acute pharmacologic rodent model of neocortical seizures. Optical calcium-based ECoG maps revealed that neuronal waves propagated rapidly and remained mostly confined to the seizure focus. Glial waves were triggered by ictal onset but propagated slowly in a stereotypical fashion far beyond the seizure focus. Although related at their onset, the divergence of these two phenomena during seizure evolution calls into question their interdependence and the criticality of the role of glia in seizure onset and neurovascular coupling. (C) 2015 Elsevier Inc. All rights reserved,