A transgenic marker mouse line labels Cajal-Retzius cells from the cortical hem and thalamocortical axons
A transgenic marker mouse line labels Cajal-Retzius cells from the cortical hem and thalamocortical axons
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DOI：
10.1016/j.brainres.2006.01.042
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发表时间：
2006-03-10
期刊：
影响因子：
2.9
通讯作者：
Pleasure, SJ
中科院分区：
文献类型：
--
作者：
Zhao, CJ;Guan, W;Pleasure, SJ
Cajal-Retzius （CR）细胞是最早出生的皮质神经元之一，是啮齿动物和人类正常皮质发育所必需的；然而，它们的胚胎起源一直存在争议。最近的遗传谱系研究和CR细胞迁移的直接可视化表明，CR细胞在发育中的苍白球边缘有多个萌发来源。我们利用Frizzled10基因的5'未翻译区生成转基因小鼠，以标记皮质边缘（端脑神经上皮的最尾部边缘），发现这些小鼠忠实地复制了先前描述的皮质边缘、丘脑背侧和背神经管中Frizzled10 mRNA的表达模式。在皮质下摆，LacZ mRNA的表达仅限于心室区，LacZ蛋白的持久性是胚胎时期源自下摆的CR细胞的谱系标记。当这些标记小鼠与FoxG1 （BF1）突变体杂交时，他们证实了先前的发现，即在这些小鼠中，皮质边缘扩大，导致皮质内侧壁CR细胞的产生增加。(C) 2006 Elsevier B.V.版权所有
Cajal-Retzius (CR) cells are among the earliest born cortical neurons and are required for normal cortical development in rodents and humans; however, their embryonic origin has been controversial. Recent genetic lineage studies and direct visualization of migration of CR cells have demonstrated multiple germinative sources for CR cells on the edges of the developing pallium. We generated transgenic mice using 5' untranslated regions of the Frizzled10 gene in order to mark the cortical hem (the most caudomedial edge of the telencephalic neuroepithelium) and found that these mice faithfully reproduce the previously described expression pattern of Frizzled10 mRNA in the cortical hem, dorsal thalamus and dorsal neural tube. In the cortical hem, expression of LacZ mRNA was confined to the ventricular zone and perdurance of LacZ protein served as lineage marker for CR cells derived from the hem during embryonic life. When these marker mice were crossed with FoxG1 (BF1) mutants, they confirmed the previous finding that in these mice the cortical hem is expanded leading to increased production of CR cells from the medial wall of the cortex. (C) 2006 Elsevier B.V. All rights reserved.