Osmolarity regulates chondrogenic differentiation potential of synovial fluid derived mesenchymal progenitor cells
Osmolarity regulates chondrogenic differentiation potential of synovial fluid derived mesenchymal progenitor cells
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DOI：
10.1016/j.bbrc.2012.05.015
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发表时间：
2012-06-08
影响因子：
3.1
通讯作者：
Krawetz, Roman J.
中科院分区：
文献类型：
--
作者：
Bertram, Karri L.;Krawetz, Roman J.
软骨是少数几个尚未鉴定出成体干/祖细胞的组织之一。最近的研究已经提供了强有力的证据，即来自滑液的间充质祖细胞（MPC）的亚群可能能够在体内和体外/离体两者中影响一定程度的软骨修复，然而，在关节炎患者中似乎并非如此。以前，已经发现，在患有骨关节炎（OA）或风湿性关节炎（RA）的患者中，滑液渗透压降低，并且这些渗透压的变化与软骨细胞基因调控的变化有关。然而，它仍然是未知的，如果在渗透压的变化调节滑液MPC（sfMPC）的基因表达，并通过扩展，该细胞群的软骨形成。在本研究中，我们收集了正常、OA和RA膝关节的滑液样本，定量了滑液的渗透压，并修改了培养/分化培养基，使其渗透压范围（264-375 mOsm）。除了使用Sox 9探针的定量PCR（qPCR）外，还用培养物的阿尔新蓝染色测量软骨发生。ACAN和Col 2A 1。总体而言，与从正常滑液中分离的sfMPC相比，来自关节炎关节的sfMPC表现出降低的软骨形成潜力。此外，如果在相同的渗透压条件下分化，sfMPC保留了增加的软骨形成潜力，它们最初是在其中衍生的。总之，滑液渗透压确实调节sfMPC的软骨形成潜力，然而，需要进一步研究以阐明渗透压变化被细胞感知并调节软骨形成基因表达的机制。皇冠版权所有（c）2012由爱思唯尔公司发布。All rights reserved.
Cartilage is one of few tissues where adult stem/progenitor cells have not been putatively identified. Recent studies have provided strong evidence that a sub-population of mesenchymal progenitor cells (MPCs) derived from the synovial fluid may be able to affect some degree of cartilage repair both in vivo and in vitro/ex vivo, however this does not appear to be the case in patients with arthritis. Previously, it has been found that synovial fluid osmolarity is decreased in patients with osteoarthritis (OA) or Rheumatoid arthritis (RA) and these changes in osmolarity have been linked to changes in chondrocyte gene regulation. However, it is yet unknown if changes in osmolarity regulate the gene expression in synovial fluid MPCs (sfMPCs), and by extension, chondrogenesis of this cell population. In the present study we have collected synovial fluid samples from normal, OA and RA knee joints, quantified the osmolarity of the fluid and modified the culture/differentiation media to span a range of osmolarities (264-375 mOsm). Chondrogenesis was measured with Alcian blue staining of cultures in addition to quantitative PCR (qPCR) using probes to Sox9. ACAN and Col2A1. Overall, sfMPCs from arthritic joints demonstrated decreased chondrogenic potential compared to sfMPCs isolated from normal synovial fluid. Furthermore, the sfMPCs retained increased chondrogenic potential if differentiated under the same osmolarity conditions for which they were initially derived within. In conclusion, it does appear the synovial fluid osmolarity regulates the chondrogenic potential of sfMPCs, however, further study is required to elucidate the mechanism by which the changes in osmolarity are sensed by the cells and regulate chondrogenic gene expression. Crown Copyright (c) 2012 Published by Elsevier Inc. All rights reserved.