NMR-INVISIBLE ATP IN HEART - FACT OR FICTION
NMR-INVISIBLE ATP IN HEART - FACT OR FICTION
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DOI：
10.1152/ajpendo.1992.262.6.e943
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发表时间：
1992-06-01
影响因子：
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通讯作者：
INGWALL, JS
中科院分区：
文献类型：
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作者：
BAK, MI;INGWALL, JS
P-31 核磁共振 (P-31-NMR) 光谱广泛用于监测完整组织中三磷酸核苷 (NTP) 池的连续变化。最近，这种定量心脏 ATP 增量变化的技术的有效性受到了挑战。因此，我们比较了通过 P-31-NMR 和化学技术在离体等容大鼠心脏中在氧合灌注 16 和 56 分钟时以及在缺氧 28 分钟（有或没有 28 分钟复氧）以及缺血 12 或 28 分钟（有或没有 28 分钟再灌注）的心脏中测量的 NTP。 NTP 含量是使用外标根据 P-31-NMR 谱计算的。在每个方案结束时，将心脏冻夹，并通过化学测定测定 NTP 和 ATP 含量。常氧灌注 16 分钟后，在同一心脏中通过两种方法测量的 NTP 和 ATP 含量值无法区分。所有七种实验条件的结果显示方法之间没有显着差异 (P = 0.262)。因此，两种方法都检测到 NTP 和 ATP 中相同的增量变化。
P-31 nuclear magnetic resonance (P-31-NMR) spectroscopy is widely used to monitor sequential changes in the nucleoside triphosphate (NTP) pool in intact tissues. Recently, the validity of this technique to quantitate incremental changes in ATP in heart has been challenged. Accordingly, we compared NTP measured by P-31-NMR and by chemical techniques in isolated isovolumic rat hearts at 16 and 56 min of oxygenated perfusion and in hearts subjected to 28 min of hypoxia, with or without 28 min of reoxygenation, and 12 or 28 min of ischemia, with or without 28 min of reperfusion. NTP content was calculated from P-31-NMR spectra using an external standard. At the end of each protocol the heart was freeze-clamped, and NTP and ATP contents were determined by chemical assay. After 16 min of normoxic perfusion the values for NTP and ATP contents measured by both methods in the same hearts were indistinguishable. Results from all seven experimental conditions show no significant difference between methods (P = 0.262). Thus both methods detect the same incremental change in NTP and ATP.