Formation and specification of distal leg segments in Drosophila by dual Bar homeobox genes, BarH1 and BarH2.
Formation and specification of distal leg segments in Drosophila by dual Bar homeobox genes, BarH1 and BarH2.
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DOI：
10.1242/dev.127.4.769
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发表时间：
2000-02
期刊：
影响因子：
4.6
通讯作者：
T. Kojima;Makoto Sato;Kaoru Saigo
中科院分区：
文献类型：
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作者：
T. Kojima;Makoto Sato;Kaoru Saigo
在这里，我们证明了BarH1和BarH2，一对Bar同源框基因，在果蝇远端腿节段的形成和规范中发挥了重要作用。在三龄早期，Bar阳性和Bar阴性组织的并置导致中央折叠，可能将未来的眼睑节段2和3分开，而在后期差异表达Bar同源框基因的组织的并列则产生了远眼睑的节段边界，包括膝部/前膝部的边界。Tarsus/pretarsus边界的形成至少需要两种不同的Bar功能，即早期拮抗作用，与tarsus特异的同源异型盒基因ARSTAISH相互作用，以及随后在靠近tars5节段的tarsus前细胞中诱导FasⅡ的表达。BAR表达需要无末端但不需要腊肠，而早期圆形腊肠的表达在内部以BarH1和BarH2为界。
Here, we show that BarH1 and BarH2, a pair of Bar homeobox genes, play essential roles in the formation and specification of the distal leg segments of Drosophila. In early third instar, juxtaposition of Bar-positive and Bar-negative tissues causes central folding that may separate future tarsal segments 2 from 3, while juxtaposition of tissues differentially expressing Bar homeobox genes at later stages gives rise to segmental boundaries of distal tarsi including the tarsus/pretarsus boundary. Tarsus/pretarsus boundary formation requires at least two different Bar functions, early antagonistic interactions with a pretarsus-specific homeobox gene, aristaless, and the subsequent induction of Fas II expression in pretarsus cells abutting tarsal segment 5. Bar homeobox genes are also required for specification of distal tarsi. Bar expression requires Distal-less but not dachshund, while early circular dachshund expression is delimited interiorly by BarH1 and BarH2.